| IED ID | IndEnz0002013766 |
| Enzyme Type ID | protease013766 |
| Protein Name |
Serine protease nudel EC 3.4.21.- |
| Gene Name | ndl CG10129 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MNYNMDEMEATRLLRHPRRWWSIGFGKRIVAISILVIIVLLFSLVYHGLVVEKIDQVQQIAALNARHQVLFNQPFEEDQSALIVSPQTLHFKLLDEDMNKDMEDSKNRRRKHMRQMLVKFRLNKKHRMRRDLHGLDLLDPVRMEANMQHLYTKLRSKRAREALSQLEHEFVRCKKHTPQDCMSAFLRMYKMAKEVTEKMEKMKAIMREQQPKLESSSMESHEQKGTFSPADLIQVTTAEATTVAVHATEKPARTKIKPSRISWIIDGHDHDESPVYTDGAPKKETTKAPWNTTQLVEITTTKIDATATERTTVESTTEKISWILDHFDKPQEILRTTEGPGQRIIRNVTTTSASSEPIVDTENTNSDHVPTTENGLVFNITTDGPVETTKSTAQRKLSFDWILDGEENVEPEVKSTNTTTTTAATTTTGATSETIIVTTELPKITFDWIIDGREVVEPQETTTEVTGTTERLRKMPFDWIIDGEEVVEPQENVTTTTIATTVAVSTTEINERIHNSTAYPTKPKPVKFDWIIDGGESSGEVSTSSTSQPKLTTREAISNPESPRSSHPLDNPTSIENMLESFEQHEEQKPILRVLNANESSSETVTDGYERQLWLKKFEDQARPNQNELIDTFGTALDAKALDKMGPKINPLNGHTWNAADAQILSLCERVALRMRNKVATMSDGETKEKGETFTASPSVQFTSRAPGGFPVSGETMKASAQFMFNPNFGMPSIPVCFYMTPANFRMPMWSNTPTFMGMQGAHFGGSSNPGAGIFFVPQQFGPSGNFFGGSGGSGAGGQGANIFSKNASPQKPTNGQQQVYCSYMQNQSGQGAGQSQTSSQQQQGGQSAFSNANFKMRHANQTNTANQQGQIIYASYAGLPQQPIQERSRCPEPDQFSCFGQQECIPAARWCDNVVDCSDGSDESACTCADRVDEERLCDGYEDCPMGEDELGCFGCESLAYSCYENPQDFAKRNRSTISMCYSRLERCDGFMNCLNGRDEEQCSMLVTDVADHMSHGASASEGYLYHNYRGDWHPVCNNGEKWAALACQMDENSRMDHSASLNVSFQTLTLPGPFIEPSLHAGVHFAQACHGRNSHDSLVDHVAYVKCPPMQCGLPSKSSMLEHSKRVRRAVSDSKEIVGDGRIVGGSYTSALQWPFVVAIYRNGKFHCGGTIYSDRWIISAAHCVINYGKYFYEVRAGLLRRSSYSPATQIQPVSHVVVHQAYERRSMRNDLSLLRLLNPLQFNRWVKPICLPDKGRTTVGDDWIWGPVEHTLCTVVGWGAIREKGPSSDPMRQVIVPIRKKCTDPEDQASEDICAGDPDGGRDACQGDSGGPLFCRSVSNPDEFYLAGVVSHGNGCARPQEFGVYTRVTLYLDWLEMATTPRLLPKLQPLQLCPGFICVWGGKRCIAKRQRCDRNVDCLGGEDEVGCTYNFLPDMVGGVRQNISTTTESDYHPVKESEEKSKMREVIPIDDEDLKAEQDEEDLLKSTTSLGQTETTQGPMDLSFAEQITSTTSDDLSITDETTSTDFTVSDSATSPSTLLPTTTNPSTWLPSTNIETSTFSFTTTESEASTKQETLPTTVAQTTTIPTSTEDLKKLTDLVTEFIESTTFETTMEVETTTLSLTSTDAPKLVTTEGVKETTTTEDTTTISSIVTLTTTPLATISTTILTTEKHVAVTTLAPTTTTESAKTTTTHSSSTHSEKDQIQIPNKFVCKKMSQIVDIMMRCDRKVDCEDGTDELDCTCKDYLKGSLKGLICDGKADCEDLTDEQNCVECQSNEFRCPLSKTCLPLSSRCDNKVDCKFKEDEKDCFALTNGHDVHFDVHQQPKFSSTGIFSRNGHGVWRVVCAHETGYHEHQAKTADAVCALLGFNGAHYFNSSEFVTQHEMQPITPELKGGRNRMSAQIHSMVGDNVQFTENEVIIPELGHPSASRPEKDRLLPRKCVGIYVECNPYSNKTTPLKTFSAGQVVKEKPIEQVPVLSPTIETHNTPNVHFKPQIPAMVVNKKDEILDRLDKLIKSKKNKTILVNEQLHEAIEELHWPWLADVYMNGDLWCIGVLIDKHWVMVHESCLSGIDLETHYVSVLLGGGKTKRSAHRSNHEQIRRVDCFEGVPKSNVLLLHLERPVRFTHHVLPTFLPDSSHQNQSHARQCISVLHDDATGRIKTVAITRIHNATNCDSCYKLQEKQPPANLMRLLNVSAEDMASISEEVELINGVAPTELPAITKFTTCNQFGLKNVSDAHHNPSDQGVLVCRDSHTGWFPTALFNYNNSDCQSFKQPFGIRTLELVYKSLQDIIDKPSCKMLLPAPDCSTHRCPLGTCLPQAAMCNGRSDCHDGSDEEETKCRQQKQQCAPGEMKCRTSFKCVPKSKFCDHVPDCEDMTDEPTICSCFTYLQATDPSKICDGKRNCWDKSDESSVLCNCTADHFQCSSSPEDCIPRDFVCDKEKDCPNGEDERYCFGIEHPLHLQKKDFWTNSQHTQPEIAPQYGQVIEQTYGIWHTKCFPKSKPPQVDEVREICKKLGYNPYRQPSYRLIDDEENKPVHTYELADRQGRSFSNESLMGKYRDSTKALIISKFSPLQLNERLTLFLKSSRPIAELVRWNATDSSMCYRLEIRCA |
| Enzyme Length | 2616 |
| Uniprot Accession Number | P98159 |
| Absorption | |
| Active Site | ACT_SITE 1185; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 1233; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 1332; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo. Nudel, pipe and windbeutel together trigger the protease cascade within the extraembryonic perivitelline compartment which induces dorsoventral polarity of the Drosophila embryo. {ECO:0000269|PubMed:7671306}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (30); Domain (13); Glycosylation (25); Modified residue (6); Motif (1); Region (5); Repeat (6); Sequence conflict (16); Signal peptide (1) |
| Keywords | Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Hydrolase;Phosphoprotein;Protease;Proteoglycan;Reference proteome;Repeat;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000269|PubMed:7671306}. |
| Modified Residue | MOD_RES 215; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 220; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 574; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 581; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 1134; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 1136; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897 |
| Post Translational Modification | PTM: Requires cleavage for activation (presumably). {ECO:0000269|PubMed:9477324}. |
| Signal Peptide | SIGNAL 1..43; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10201075; 10449356; 10602463; 10625559; 10628985; 10696585; 10731134; 10922064; 10961941; 11051547; 11076773; 11283698; 11296245; 11562344; 11700289; 11744369; 11819114; 11973283; 11984873; 12068947; 12417410; 12493753; 12568721; 12871909; 1339676; 1425342; 14711428; 14731500; 14731544; 15166158; 15324670; 15788537; 15840717; 16260500; 16271864; 16515779; 1672778; 1688006; 1733499; 1742496; 1794309; 17996400; 1814368; 18776742; 1899484; 1904007; 20220848; 20605458; 20638387; 2068782; 21074052; 21775776; 21930794; 22751930; 23071443; 23550122; 23632253; 23868318; 24255095; 24711428; 25124754; 25294943; 26173873; 26979755; 27172210; 28179368; 29670218; 30146479; 30367934; 32143742; 32901612; 33827210; 34343463; 3653004; 3931918; 6434989; 7527496; 7663024; 7720559; 7950316; 7953570; 8026333; 8124709; 8139688; 8178370; 8241768; 8698232; 8749385; 8825480; 8844153; 8913032; 8970732; 9420336; 9568717; 9598341; 9694625; 9735365; 9827795; 9827800; 9889092; |
| Motif | MOTIF 1031..1033; /note=Cell attachment site; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 292,492 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |