| IED ID | IndEnz0002013862 |
| Enzyme Type ID | protease013862 |
| Protein Name |
Replicase polyprotein 1ab pp1ab ORF1ab polyprotein Cleaved into: Non-structural protein 1 nsp1 ; Non-structural protein 2 nsp2 ; 3C-like serine proteinase 3CLSP EC 3.4.21.- M-PRO nsp3 p27 ; Non-structural protein 4 nsp4 ; Non-structural protein 5 nsp5 ; Non-structural protein 6 nsp6 ; Non-structural protein 7 nsp7 ; RNA-directed RNA polymerase Pol RdRp EC 2.7.7.48 nsp9 ; Helicase Hel EC 3.6.4.12 EC 3.6.4.13 nsp10 ; Exoribonuclease ExoN EC 3.1.13.- nsp11 ; Non-structural protein 12 nsp12 ; Uridylate-specific endoribonuclease EC 3.1.-.- NendoU nsp13 ; Putative 2'-O-methyl transferase EC 2.1.1.- nsp14 |
| Gene Name | rep 1a-1b |
| Organism | White bream virus (isolate Blicca bjoerkna L./Germany/DF24/00) (WBV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Tornidovirineae Tobaniviridae Piscanivirinae Bafinivirus Blicbavirus White bream virus White bream virus (isolate Blicca bjoerkna L./Germany/DF24/00) (WBV) |
| Enzyme Sequence | MSILFGNRQANATKRSDMASVARAVYEVDLISTKYARRTQERLAHNKHAKPSYPSVFFGRRMKAVKEPTFTPSTLFFEEATLPKVLASKAKPDTGIKTRRVYVADSLTINGHTYPIVGHFVEMAVSKKEAFPIQPKRVKPKPLMAKPIPNIRRTFLTPEERTNTPTTPTTTTTTPFVAGETAGPTIEYTPTSIDLPFAMPTVKQIKENAHTILREQDDCLRFAQTALFKHLGTVTHTTPNHATTFQVKGRTSLLTFEWRKTTQSPLTDGHFYLQTANNHAELMQPVEGKLTTIFTTTIQQGTTHSLHLIKQESARTLKTRKPLKLVTYKQETPTTTITPQSLKKTITYIPGSFCINVAEPTLQSVMRRQPLTPTPNDALLQIYHKLGCTTKSPNHASTFELFGNTYTWYPVQHTNNLLHKDPNRRFFLHITGQTPQLLIRTERKTFLTLQDEVTYISGKLFVMNHAPIQGEYKTQTAEWVGSYNMAKTPKAIKPAKTVEYINTTPCHKPATMPAPITYRQCPYTWTLHEPSISKVQRNLFHIPKTATNCLDRIQKALFPEIVTSNQHFPIGFTIQTDTTMQSYEWAILCKKVTRTYYLAVQNHHATLWYKCAQVYMCLSDDIAPTTELQGSVYILNKVTDPGFYQNTRQWCGSNDDLHEPKHLIKNLANGDVNNIAHCSLTPWTTTPLVYSTQKNKLTRKLIHYYNATYTVQVPKENPNAQPSTMKCAYKAYIDLATPTELQIHLDLEIQGKLYSQTAQKKGSKFNKLDIPTFGDILKGTLYVFSSKVLLYEQPKRCTSVCFHLPNNAQSFFFNTETIQTFEDLFARISSEEVEDNLVLIKGFVPCLGAIYITKDLKFIQPELKEDFKHPTTYYTFTTTVDPEQVLYSLHPSFTDIVPPHGFPYYTFAKLNNHIDAWNITDDQADTLAEAQPIIFQWPTEEATITTPYKVLHYEHLEGLDYISLSSFNTVECPEETQSAHDSSSESESEDEELPAHPLSNAPSQASLSSVASTAPPTSPTSSPTPSPTPLQQQVGLKGKEVPVGGWVLVSEEETPSEEVDSPKLLPNEVPLSFDFDLPIEPITRPISPELQQPILTHYEHPTSPTPSVEIEIDFGSYENLTLQTEETVTTEVQPEPTPAPTPEPTIVTETVQETPVPTETTQESTPESTPESTPEPTPESTSESTLEPEHVATPSQSPTHITVTEITHEPETPDSWSERYDSTSNIPEVFNQLSFGSTDSVKITTPKTETPDEPQQPTVETVSAAQQLLQIVQTATPDIAQLMSELPPYRLICIGSYCPILAENISKQLPTAVTTPTDADIPTVIFNVSEETMDTVINTVKTKHQANHLTFSLTTIIALDVPKDKSLPLQQIYDKLTQQDYNTDFIYESHHRQPKESLTHASVLSAYTASYKTTAIKSIADNAVVLDIGYGKGNDGPRYAVRPLTVTGIDTAARMLAIADQNKPENVTLVKQGFFTHITKTSNTYTHVIAFNSLHYPLASSHPDTLVQRLPTCPANILIPCHHLLEGIQTPTYSVVKDEDMWCVKVTKNEFIESSYNYDVFVKALESKYHVTIGSLLDCVEKPSTRSITPTLWTAMRNFVNNDQEMQRILSGYITFNLTPLPPKVEIINDWLDNNATVTINNPFASNEGVTFAVHNIGAITTTEGEFIVNAANKQLNNGTGVTGAIFAAHDKELKLTQAIKALPTYGASDKLESHQHVVQTIIKNNNSTHAINILHAAAPIKVKCTSKNPEVLLAHNETAQSELKETYKAIVDYAQLNKLTHIYLPLFGAGAYGHKPLDSLEAFLDAMRNRSPQSTTQYTLLLSDPVKPLDNPSFSYEFLNLLVTNLNINKQFAQLIVNKYHNTCALQSAIQMNTTTDTHNFLATFIYLLYTMPYSMNTFRQTHTPEEPFTPGKMVTVVDTTIAFLTTLDILPPCGQPCGYLPPSITKDGEYICACQKTSNWSLPFHFYNARYNKVYHTGLNNILTHKHSAFHKSRNAAHFIAKTGPSTSSYPVYMAPVPEILAYNASYRDSCQDNAIEEQSDSQASQSPSSPVTIPVSLPTASPASSVKSALRSDIPITTDQQSTTSASISTATTASTIPTAPLTSSDSNTSVVTSLYGNMEELTYLDASGTSQDFILSETTPFIAHIYHNNEATFIPPGYQLLDTNTNDPIEMYITPPRPIDGSPMISLASTASTTPMTYPLLSIRLTTEELTSFFKTKTDKFHLISHKSCLTVHLFDSPTLNSIAADSTSDAHLYQQHLKDLYTFSDCCSMYTRTEVYNCIEADTPLIRQSEQTKFHPINLDTLIEMVATFPPIVKRYSQTTTPDFTNLTVYFVSNGDIITTPTGSTSEQPPQLKIFLDYQTSSKFTTLVDLTLHEQTEANTIITYHHGEHQLLKPNPSAFYIEFQTYSSFFSRFQTFSTNFFWTLFINFLINVRFCITADSAYFHWQGKPIETTNLNIVYSIGRLDFVLSKHTTPWLTKPTDTLNPLTLIKNTLVQPIAINFHGRIRPLQSTNTRFGATHTPTKLPVHLLNTSLRTHYLSLLSLFQCTFSVFLAYIALLYSFSGHGIFTVVAYFTMLFARYYITSFINFCTSQLTATQVTQWFAAIKAKYTGIYESSQDRVLTVNVTGTNVPYIVKYSTILTVTMYVAFMAFVWTVSTYAAQYTAGERYDRPPYQTVFQKTLNVLGLTETVTYYYPYASLNEACAASTSILCRLGSPFNFHYPSDYTQVRTVQTDTSSPFWLFIIFMPPSFLFIVLPWLILCTITPTVSIAQLLVPSIILNATIVFIYIRRKFTGHCCGPHTCIKHADISRSLQFRPTSQIQHSLTFCGTLCAKHNWYCNNSDSPTHTLGIQLAQLIETTYKLQPGTIKPDSSYTHTTETATLPIMKVSTTSTDFTTSEPNTTVEHLHLQVIAHVTGTRISIESSSNKVQQQNTQHTRLTNKPVTGFMHTTLLQKLKRQHKDELSSYLCNFVPSDNKKDCILPHSVVHMTLTENQRTFLLKNFTFSTNVTVDPTTTGFIPSSLNISTLPHKHFMINVIESAMLAKLPKEVQDTLRTTHLETTTLERQAMSLTTQAILTTFALIMATFVVAFLAFFSTAQVGKTPYAGLNPTMVGNVNAEPYIQPTTLENSILIPLHGASKVCWRAQNGTLFFTDAIPTTECARAAVPYIGYKSEFTQTCASSNLRYPFTVYLGSIKVMYLRDGISYLTSTLSHNSNTKKLCVQVGSNAVRCASVLPTGASSNVAALLMASVVVISMVLFYLYLLQIFKFYTNSVIMSFVIQLLTLLATTVSTPLAVTVQLFVITYGYTNWILLTLSLLNLTVLLSTPVGITFVVIYGLYKAYTLFTSSGQGCVYNEGGTIRFSGSFEQVANSTFPLTNASCVQLLSDLGITYQQLNVYASSRDRNVRRLAQALLHRQLDSASECILYEGCSGNTITRQALQRIRQAVTVVVTPASQNLCKITSNQANGIGLSCTGTFFTSTEIITCAHGIGTSDITAVHKGITYDCKVKSINNDIAILITTTVNLSVQNIKLDSSFSQKSDNYQRNFVQFVSFVDQQNSDAVTINNTVMLPSGHFFAIGTEAGESGSPYTLNGNIIGIHYGIDNAGSWMLASRPDGSFYVPATQHGNSAKVTFSTDAFAQQFPAIVTNKTSDQLVNEIAATNNTAFELDTTDVSHLSNLLKHLKNNNETPKSLSDYLPVAPVTQQTSVTVGQTLTSPVNNMQTALYTLMLVSEVITYILTPNSDLSVLISMFVTSAFLKFGASKLFYNTEMLRNTITTFVVYRYTTLLIALVFSQYYLHILSYALKLNTLVLTVIALTFLVTPLVLLTIRRVYYYSQNYLISCVFIISCFASHTYTLYILTDTTVDFQTFIISEPIFSTLLCNLFIGFTLISVVPNPLYVCVVFMYILLDCEALGFIVTCFMASYLCPKPLRSLTTFLCTDTLVLTAPAYLHWYGAKGTQREYSVIYAVFDSILTPETTIQIPVTIMEGIEQQVKFIFAVPKSANIQDEEEAYVEYNQNSDIKDLAIKNEEKVCTITGMFRKTRTIKGTLMESFYPRHQPDSYILNQVVRHNYVLAHDPETIILTTVNPTHLESEPFQTIVKKVRKLHALYQEISEQSSDDTELCHAYILALIKSTVLEAQMPTEKINFVSSQTMLSPAMILIFAEAYQILTESRSFTNHITPQSDIGSMQTTLASLAEMDTDEMTPQERKIHIKRMNVLKQEIAKMESASLKLEKFLDNMHKAEISKRGKEDILLKVSNMLRLHLNKVANAAHCTIQTPSAGLITLASAFDVHSLCVTQHSESVLIQTPDDDTFLVYVDGQIYTCYNPTDITGKKLVPINVMSPDNVQFPTYPVVFSLSKQDYAEEITEQNNIGYTERTHNFKLKELASGLAVTLDGLVVVTETKELATAFKIGQRYFKFLNTNKTPVARNNTHAIIQLLRNNISQQAVVRIGGSRVSNDHIAISQVPVQTIGYLTYAGISVCRQCATKQDHTCQYAGYFVQIPREHVSNIFNLTDTPPCLHNKFTCTTCQPLQQQSKTQQPPLNLVGQCLGLTLDTAFCPFQTGEYKPSSREFYINILNNNVASLRKVFKKNTASIPSENGTIMLKDTGTAHEIYVAKQLLAKGLPVLQHARFNHDGTDYLIRYYTTPYSLGDLVYAYMVGDFKHMLLALDITDETCLDPGNYSSYYNFKEQLRNKLASVIPNVNKILAAELPLAITLDNIDLNGFLYDFGDYPTNGKVTNYHVVSCMRQIATFCSLDITQFPSPLGYTVDRPKLQQTLITGSYIDKLLAINALVASNPETPATASTLFIEASAPTTQTAAISNPILGMHVLDWDLIKANHTGVELDLIQTQDPSIYAKPDVLSVGDTIFYYGRRRYKHDAYKRPFYDLDLIQRMNSAGLNLSETTGYHYQCGTTTEAVEDFMYYNYNSPKSFDPSYLKSVYTYMRDKFMKIISTDEKLNHQSGAPRMSSMGVGVSGFFQKTVWNALPEDFSPRLLDTASKTVMPFSTNIVKKFQRQKKTRVRTLGGSSFITSSIFRMLHKPVTNKMVQTAQANIGPFLIGISKFNLGFHKYLSAHHPNGIEDCQVMGADYTKCDRSFPVVCRALSAALFYELGHLEPNNHWFLNEMFAFLLDPSFISGHIFNKPGGTTSGDSTTAFSNSFYNYFVHLYIQYLTFLTTEMPPSYQPLCNLAHQAFSTGNTETYDLYFSMADDLNSTEYFLHFLSDDSFIISKPTAFPIFTPANFSMKLQNVLGCYVDPAKSWSADGEIHEFCSSHICKINEKYQYVPDPNNMLAGLICAPEPTPQDKLIWKLVATCAELAVFHFVNPTLFNNIFHLLQSLHAEFVSEHSVNLLPPKLLEIDFYTDLIDSEDVEQYSFLADTLTEKNIIMQSASQCYFCDNATVSTCSDCTVQYPMCAHCAYEHLMLTDHTPTQVLPCHVCDQTDPRHLNHTFVMGTVKVACDNHVEGMALPLVDHARKLVKIPLYQKCEQQKTSVSAIKYTKLFDENDQPLDPNFFFYDHEQSAEHNYLKILNDCYLLDEHTVQTSTTYDFQCLEGNTIQVFHKPAETFGNTAYAEILDSKGRVVLKVTLDPISAQNPNHYYITTTKGVLYRKYSKIRRTIHKPRLANRHILNTLKKATFIIGPPGTGKTTYVMKNFIDTASPANKVAYIAPTHKLVQSMDQAIWDKYNHTVSVSVVKSELNNNKYNYPLNSATKTIMLGTPGAVCTHAGCTLIFDEVTLSQLNTIINAISVVKPSQVIFLGDPLQLGPVTHMRSLSYSYTNFPLFQFCNDSRVLSICYRCPSNIFNLWVKPYTDSNVRIDPHAAGGDAKIIVSDQCSNPDAYQYVQKLARNNPDKVLLCNYKKPIIGLENAVTIDSSQGKTYKHTIVVLLGNTNFTQVINRAIVAMSRSTHSIEVHCSPFIHTKFSELFGWPQNVEKENQITKQLHEYSVTSNITLLPVSELPNHLGSLVVCDLEFFHVRHETTPKVKCTLEVGEMAIITTSLLKQIIIPRKSAFTAETHHKHTFGVPKGKPDMNWDYMKSHKAISQQINTDRTHKIFSHMAATTLNRVVYVLYGAGNDLRALTNLNIVGDYTCEKCTKEATFYTIHREIVHTFCNHHAPSQFPLMGTINAQAIDIQHASANKQSLTNTHAEVCNQQHGDAHTASADTIMTGCLASNFLMKSAIKLDNILTTSAFKPYAPYIQTGSHLTVSSIKFRTLGTGVFSMIDDKLYHFDILPHHSKLSHFLQHSSNQPHSTIVTEIPAGYPSCVKIKGKGCTFCANTIAVITELYEDLAKLGLTLSRPIIQQAYTQVETQILQNITNVSYNQFGEMLIQLKDDTVIPFINDFQTSIHNYSLRSNQPLPNPAIFKNLGIKATLGFSTPWVPVTTTTTEPHIMSTKILKDNNDYYHLSPVQLKASPHAFSSITAGYYIYTTPMINIPNETPAYYLTHFVHGQSTPLDLGYTSTNRLTTKQFIYTPNEDEYKSKLGHHVSVGDTSNVSWTIGGMHVLTAFQNITNYQLVSGPANPIMRINVALERGNKVETTALDTTLQDYYKIATTNKVTVSKTTFFTLDGGQYRMMNFANPDGTIQTSYPVAQAQSQLITNYRIYPSYITWPTFFTNEATDCWAIPNYNAPPKNQTCNINIQKYDQMCDLFAIDLKIPVKGHIHHLGNAGNKYSPGDVVLRQYFDQAHLTSYDLREVVSDIPVLHPNDEWKAHFILSDVYAPDTDFTSLALEYMQNHLRLGGSIMWKMTETSILQVNEIVKYFGSWKAVTFAVNYSSSETFLFCAGYTGVEYNTSIVQNGYMSLLGGYRKDLLFVPFCNDYTGSKAYKDTGRVKVVANHLADKLTPAHYATASIFLNTALH |
| Enzyme Length | 6872 |
| Uniprot Accession Number | Q008X6 |
| Absorption | |
| Active Site | ACT_SITE 3492; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000305|PubMed:21068254; ACT_SITE 3518; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000305|PubMed:21068254; ACT_SITE 3589; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000305|PubMed:21068254; ACT_SITE 5980; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 5982; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6086; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6169; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6174; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6497; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6514; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6547; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6654; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6729; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6758; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6790; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 3.4.21.-; 2.7.7.48; 3.6.4.12; 3.6.4.13; 3.1.13.-; 3.1.-.-; 2.1.1.- |
| Enzyme Function | FUNCTION: The 3C-like serine proteinase is responsible for the majority of cleavages. {ECO:0000250}.; FUNCTION: The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. {ECO:0000250}.; FUNCTION: The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction. {ECO:0000250}.; FUNCTION: NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 5649..5656; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (15); Chain (14); Coiled coil (1); Compositional bias (5); Domain (9); Metal binding (16); Mutagenesis (6); Nucleotide binding (1); Region (7); Site (11); Transmembrane (16) |
| Keywords | ATP-binding;Coiled coil;Endonuclease;Exonuclease;Helicase;Host membrane;Hydrolase;Membrane;Metal-binding;Methyltransferase;Nuclease;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Repeat;Ribosomal frameshifting;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Non-structural protein 1]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 2]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 4]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by its own protease yield mature proteins. 3CL-PRO is autocatalytically processed. Two distinct N-terminal and C-terminal processing sites can be used, potentially resulting in four different processing products. The preferential autocatalytic cleavage sites are displayed. The polyprotein is certainly cleaved into more products, like in toroviruses. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 769,510 |
| Kinetics | |
| Metal Binding | METAL 5401; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5404; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5412; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5415; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5422; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5425; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5429; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5435; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 6101; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6104; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6122; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6125; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6157; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6161; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6165; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6180; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298 |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |