| IED ID | IndEnz0002013872 |
| Enzyme Type ID | protease013872 |
| Protein Name |
Replicase polyprotein 1a pp1a ORF1a polyprotein Cleaved into: Non-structural protein 1 nsp1 ; Non-structural protein 2 nsp2 ; 3C-like serine proteinase 3CLSP EC 3.4.21.- M-PRO nsp3 p27 ; Non-structural protein 4 nsp4 ; Non-structural protein 5 nsp5 ; Non-structural protein 6 nsp6 ; Non-structural protein 7 nsp7 ; Non-structural protein 8 nsp8 ; Non-structural protein 9 nsp9 |
| Gene Name | 1a |
| Organism | Breda virus 1 (BRV-1) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Tornidovirineae Tobaniviridae Torovirinae Torovirus Renitovirus Bovine torovirus Breda virus Breda virus 1 (BRV-1) |
| Enzyme Sequence | MSKTSRELTNETELHLCSSTLDLISKSQLLAQCLGTPQNLVSLSKMVPSILESPTLEPRYTSTHSSSLQSLQLLALNTSSTLYKWTTGSISKLRGHLERELCRGLVPLNDFIPKGNYVELSLMIPSVLTGQGTSTTTTLQEMCSDMVQSCIKSMETDLLKGVLALKDQTSCQEYFLSANYQSLIPPQPLVNAMRMSSVVDLSPLILENTRLLLKLSPFHGGTSVSYTSMIREFVDCSRRDEKCLKRRLTKKQKRQEEGSFDANKVITLGGKMYRYRVVILKCSDEVDDLIGFDGKVGEFDYNFENVPHCWRDLVKRRCLIRAKATWNLAGGVDENLDHVYIDESQXDFRCADGSSDSPSACVEDPHLEERIFSRVWLKQTSRFFGTKIQQVSELFKSIGLPELETTYCGVNPVKVGNKWLSFRDQGRSRVFFVYTDSNVYLATTRQKVCCDYILTKFKSVKWIGNKPDQCRVVKVLAWLISVNKVKNCTRVITPMLTVQGKISHRRVDYLDISVLDSYVSDTAGLNCVQKVKKFLSMYYNCGADLGLLDNFLTPIECGTKQLVFERCNCPNHQFYVAQFDNHVVLGLGRPTGVVYPEEIPSCANIYAVGFATQKRVVEVHYYSEMDRHQLPQDYYYFAYDQEFQHVGGDDYVNHHLDDVEDQPFPPVLFDDVYDSGDSLDDGGSDLDCFDVGYDFFWPEAPIPVPSPYGYYQGQRLRDLCVAGGDFGCDCPRCDGTFIYHPFRPRHYHSFDEVGPFIQMCEFTLTYSGQNYNLFYGLEPKVCLQDLVEASDKLLQLLVRGQLENISLPNDILACLSSLKLGANIHPFLWPAPFFNANGEWVDIFGGGDFTVFGEDFCLKAKSMVESVYFLVENFFSVDCPIGNLYCNLHLDGDVKKMLWSTIHMKYIYLALIHSEKVFNIILNSRQLSHQELVKLVIIGTFDVSIVAPCACSGDCNHGKVYNWTNLLSSVYRFVTLDQLVGLSYCEKRSLVLRKVQQYLEVEEGYQRPVQLLMAPFYGFNDNAEPDEQPLTGVFHQQVMQMFDTCVMLDVICGLKRPRASVYNLFGVLADYFRRPFTFRYYQVAEFSGSESTQVFTDVTSALTSKDPCSNRPYIYHDYAVCRVVEPRTAAVTTRGAIYPPEVIEMIRSYLPIEFDVGVMNYVDGNCDFKYCNLEFCLSGRGLVKLDTGELLDYKTNLFVVRYKTLPLLYVTSNPIYLSDFSLDNAVCLTGDFKLSFDVEPGSTLFGLYFTNGRCYRDVWETLPRFGLGTLSPPKCHSKCEPFENLAEVFFFKRRVQLVPLVNNYTPVFRHRPDIPKVLTVELMPYYSSIGYQGFVAPKCVLPGCVATQYCKLRHQLDRCVQVTKLAVAYAFYFKPLNIGSLYHLDPMRGTSYGKPAVVQFEPVGLIKEVNILVYQFGKHVAIHYFPECPTYVAYGHYPSHSVGVWLGYLPSVEECVIAQRNYRVYVPTCFRLSRTGCYHIQQDEDFERTHITVSYHYARDFDTKSLTPMFQMFSKIFGKSKQDLICALNSLSEESQSVLTLFCEEFDSAYTLQTISDEVSFETSTSPELVACVLAYAIGYELCLTVKTDGECESLDVGSSLEQVYVDYDVSKNVWDLSTHLQDDSSDDLELPFNQYYEFKVGRASVVLVQDDFKSVYDFLKSEQGVDYVVNPANNQLKHGGGIAKVISCMCGPKLTSWSNNYIKQYKKLGVTCAIRSPGFQLGKGVQIIHVVGPKSADSDVVNKLEASWRSVFQNVKPDTTVLTSMLSTGIFGCSVTDSATTLLSNLVDLDKDVVVFVVTNVSDQYIEALGVVESFQSAHGLPNFGNTCWFNALYQLLKSFAVKEQIVQDLVNCFDDFYECPTRQCVEWVCDQLGVVFGEQYDAVEMLVKIFDVFKCNVRVGYDCLARLQQVALGSCREVPADAVLMFFGQDKSGHWVAARKVCGVWYTFDDKVVVKKDPDWSKVVLVLRERGLFKATDFETPRPRRRRVAYRVPRDTISQDAIMFLEERQFSSGTMLAHSCVESVESFHVEGVQPSPLQSVDGLDDVADLSCDNHVCDNSDLQEPQVVVSQPSEVLTTSMSIECPVLENSECSVETDLNPVCEENEQVGESGIKEQDGVTTSDSQQVFSKSLDPIIKQHEVESVEPQDLPVFSQQPQVMLSMTWRDVLFQQYLGFKSDLLSLTHVNKFKIVVYLMVLWFVLLYCFSDFSLLSRFCLYVFLLWLSHVVLVVKKLDLGLVNSGGESYVLRILSSVKVPNCIAFNCDGVHWLILKLLFYSFHFYDFFVKTLVVVFQMPQLRCFTWPLLKLGFADTFLSHHILAFPTKQVSQSCLPVFGDERKYIYVPYWCKESFRTLVARAKQLTATGRTKTLDNWHYQCCSKTVKPSSCFNVRDFVFDDACNNHKHYGFFSALWFYVVFYSGFVSFWLPLMFCYCALFMCTFKNLPVNITRPIRWTVLQQVVDDLLSIITKPLFGRPACPPLSAYLTATTADEAVRASRSLLGRFCTPVGFQQPIMNVENGVAVSSLGFINPLMWPLFIVVLLDNRFVWFFNVLSYIMLPVFVIILFYFYLRKICGCVNVKGVVKNCTRHFQNFSKPLVAAGVHGNRTNFTYQPMQENWCDRHSWYCPKEEHYMTPEMAMFIKNYYNLATSPMADTIWCDYVKSVPNMTWANFKFSLFKSNETVMCGPSSHADSMLLSWYAFLHGIRFAVNPSVIDIPSQTQPIYVSSDSDDSLDKGCDVSLRPTKNKGKFKKQSVAYFSAGPVDLWYYVMLIIALGAIFVFMYSCFMVGQYVVMPRDKFFGVNPTGYSYVNAQPYLHASPPVLRNSDGMVLATPLKVPSISYSVYRLLSGHLYFTKLIVAENECTPPFGAXRLSHEFTCNDFTYILPAHLRIFGRYIMLIHPDQLHMLPFEVEHSTHTRLCYVTGTNIVECLPTFEIISPYVFVVLVAIFTIVFLFLLRMYIVMYSYFKVFTYVVFKLLFVNTVMVLFVVCLPPLVPGVVFVLALWLCDSVVFLLYLAVLSLFILPWFYVMLFVLIVGGFVFWWMMKSSDVVHLTPDGLTFNGTFEQVSKCVFPLNPLIVNRLLLDCRMSHSDLVEKSKLKTTEGKLATEMMKVFMTGETAYYQPSNFSFQSVFSKVVSPFTLHARPPMPMFRLYVYFNGQCVGTTCTGTGFAIDDSTIVTAKHLFECDDLKPTHLSVELSCRSYWCTWKEPNVLSWKFEGENAYISVENLRDFYGIDFKYLPFQQIECEFYKRMEAVTIYSIKYGSEFATQAWQTVNGHFVCCNTEGGDSGAPLVWRDSVIGVHQGLCDSFKTTLASDSKGVMMTEVKGYHVDPPVYYKPIIMSAAYNKFVADSDVSVGECTNYHNFVNEDFFSMHDELEKVSFGDKMFRYCQSLPRYLEPLHYFHVPSFWQPFKKQSVSSNVSWVVENLHFIFSVYFLVCDFVAYWWLDDPFSVVLPLFFVVQLLSTVVLKNVLFWNTSYLVTLAVTFYVHSEVAESMYLLGLFSDQIVNRVGLILVVSVMCLFVVVRVVVNVKRAIFVVVVSVLLIVVNVVLGVVQFNSLVAVCMFDIYAVFAALLTPQPVVAIMMLILFDTKCLMSFAFVVIVLSFRVFKNYKFVRVLHNLCNFDFVLTQLSLFRYRHHNQGNNPSHYEALWLFLKELYYGVQDVKYEVFSPQAGTYNVRFLTDMTEQDQLEAVEQVQRRLQRFSIVQDKNSQRLVLYSKNVDFLRSQIQHQRVLGANPFIITTLTPKDIAIDNVEVHNPSQFKPEDLQAHMWFYSKSPIFVGQVPIPTNVQTAAVLDTTYNCQDLTADEKNNVAANLQIQNAALTLSLFEECNRFLESELGDVPTLMWQSEDVVDVKQLEVQIEKLRVVLDGMQLGTSEYKATRKQINILQSQLDKALAFERKLAKFLEKVDQQQAITNETAKQLSAFKNLVKQVYESYMSSLKVRVVESNDASCLLTSTDLPRKLVLMRPITGLDNIKIVEKANGCEITAFGDTFTTGLGSNLAGLAYSSTQPLSAYPFIFNLEGIFKQQANIGYKTVECNMSSDNGSVLYKGKIVAVPSEDNPDFVVCGKGYKLDCGINVLMIPSIVRYITLNLTDHLQRQSLKPRRRLQYKQQGVRLGGVNLGEHQAFSNELISSVGYTTWVSSTVCTDKSHKHPWFVQIPSSEKDPEWFMHNTQVKNNQWVVDAKPTHWLVDADTNEQLFALALTDEEYLKAESILAKWSPITQDVECWFKDLRGYYTVSGLQPLWPVCPKKICSLKIVPIFQSQSVAYADEPTHFLSLPVVNKNFLEAFYELQEGFPGEKQVAPHISLTMLKLTEEDVAKVEDILDEMVLPNTYATITNPHMMGQYYVFEVEGLQALHDEVVSVLRQHGIACDQTRMWKPHLTIGEIKDGSVFNKFKDFGITCKLEDCDFVKLGAPKANARYEFIATLPVGDLNC |
| Enzyme Length | 4445 |
| Uniprot Accession Number | P0C6F4 |
| Absorption | |
| Active Site | ACT_SITE 3184; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 3222; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 3291; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: The 3C-like serine proteinase is responsible for the majority of cleavages. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (10); Domain (1); Region (3); Site (8); Transmembrane (17) |
| Keywords | Host membrane;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Repeat;Ribosomal frameshifting;Thiol protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Non-structural protein 1]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 2]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 4]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by its own protease yield mature proteins. 3CL-PRO is autocatalytically processed (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 505,933 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |