| IED ID | IndEnz0002013891 |
| Enzyme Type ID | protease013891 |
| Protein Name |
Replicase polyprotein 1a Cleaved into: Leader protease L-PRO EC 3.4.22.- ; Methyltransferase/helicase EC 2.1.1.- EC 3.6.4.13 |
| Gene Name | ORF1a |
| Organism | Lettuce infectious yellows virus (isolate United States/92) (LIYV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Martellivirales Closteroviridae Crinivirus Lettuce infectious yellows virus (LIYV) Lettuce infectious yellows virus (isolate United States/92) (LIYV) |
| Enzyme Sequence | MALEVESAFSLSYLLKENKQTSNNLFSLIQVPYTKMTHAKGKKTHLLRCILGSPFETLTKSSIRRSLKNLREVCNLPYCNFKKIPKNISYKYQNPFLKLYEINNNFINCKVGATGNTNSTYDWRNLSTIGNGWSVTSYHNDSVSRLHVSMVNNIPNSNTKKLSWWVTRVRSKNPWKRPYKVIYQRDFRDDGIKPYVSEFILYAPKNRADLEMQSILLSSVECFQDIKYALNYWTNLKFKEICNSALKRFTQTNIIITPVKPRVVSVNKAREVSSSVEVKLQRYEREGEVFVVSKAGKEDTRVLNDDNAIRNLFNATLNGGKYKLHPEAKSATGKRFKYYKDGFCWLDVFADANRRIPEWVKPHCLLTGSVLMSCGLWDFAKRKMVSVSHGLLHYDRKLERSSARAGVRDFVGASNEAVQREDFRDLDLRVEEFAERVLETANLRSDNRLIDNILTRASDYINKKSKESKELDINVCLSMDEKKMITNLFPDIQMSFNQKSYSNHGVFNAMRACENFYFSRKFKNSDYIDAGGDVVSTLRSKNHNVHICSPRLDLKDAARHIQRATVIDGLKGYGETISFCTNKTEDCAVNRDIIIAVEVYDMTLRDMAKAMLSHGSRKFEFSCIIPPEIFTKECNVELYEGRLKVTRIGDNVEYYYGSNGETFSHSCQTLKDILSVQVFQFGGRVFKKTLEHSRGQLHFFSICICEKIEPGSVKLKTYYQRSELDKVTLRIPVKDSFGVVTHYIIKEDREFVSSMIEYVANTGIKIDDKMVEWTYSQYRAKKTVTIKSGKVTQKETRIRKELIPGFIAIIMSEGIRAREKTHYLAKMLYTSHYKPSIVNIIFRLIMHFLGGTKRFIYESLVDCLKFLTNSDYIDTIVNTESRIEDLDKWFVFEQNVTITTDAEDQPSILEQSVKTFLSKYSEDVYERGDRSEFELEEFSSEQDLVDQLLNSGGGSKEDEWFYSYIFSIVRTKCTLSLAWRWTNRIFNFITSCRAKGKDFVKYIYEIIKIIINKPWEALCHYLVQGYKTVLKIPMTCVDSVNYLKRKSLEKILSYFSHERADDEYIFPGYDSDFSEESGDYGDENTELDSRSRCTVIKSKVEMLFRNIKGHIEKFLQRCGIIDQYNKIYHWFKSILTDVDCFNSVLNFITHTGCDALLAVLTGHFSIASCALKFMTDVVIEKNLSEKHHTTTKLIAGNLTQCIYKLDFIHPIWIPIRWGVKEISKIHIKKKLLNWRSTKEVTNESICKDLVHKSYIKYFDIKKAVWLLWFSLLIIFLHPTLGFFILFSIYPAIEIKRYYNNVVCFNNIKMSYPHVIDRLGGTYNFAKLKKAVREKFSENKSQGVKISEIPAEKDEDVMPPLEEVETPKRSVGRPSSKQDDIEDECNIKEDPTESLNFSNIEATSNRYDTLRVGNSKLCKFLRFYPKSKCYANIQTGDLIRDSIEEFYHLERSKLDIEISKMQRVVEIMNDTGRMLDNVRRMIDDRSMYVTLDGVSWYRLGCKDKNPAKEDFKAIFDHNFNIMEGNAKVKEFAVSSDEWRGMYSNERCRAIEQLFNDNNEMVRRPDVNGLKFYNKPPGAGKTTTIAKLMSKDLKNKVKCLALSYTKVGRLELIDKLKKDGIEKPEKYVKTYDSFLMNNDNILEIVNLYCDEVFMMHAGHFLTLLTKIAYQNGYCYGDVNQIPFINRDPYTPAYLSREFFRKQDLNYDTYTYRCPLDTCYLLSNLKDEMGNIIYAGGVKNVNEVYPTIRSLNLFGINVVGEVPVEYNAKYLTFTQDEKLNLQRHIDSQGGCRNAVSTVNEAQGCTFSEVNLVRLVQFDNPVMSDINQFVVAISRHTTTFKYFTPHSRLNDRVSNAISSLQSVSDFVLKDYHFRQCL |
| Enzyme Length | 1873 |
| Uniprot Accession Number | Q83044 |
| Absorption | |
| Active Site | ACT_SITE 344; /note=For leader protease activity; /evidence=ECO:0000250; ACT_SITE 393; /note=For leader protease activity; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 3.4.22.-; 2.1.1.-; 3.6.4.13 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (3); Domain (3); Region (1); Site (1) |
| Keywords | ATP-binding;Helicase;Hydrolase;Methyltransferase;Nucleotide-binding;Protease;Reference proteome;Ribosomal frameshifting;Thiol protease;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 217,266 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:13065 |
| Cross Reference Brenda |