| IED ID | IndEnz0002013941 |
| Enzyme Type ID | protease013941 |
| Protein Name |
Peptidyl-Lys metalloendopeptidase MEP EC 3.4.24.20 PoMEP |
| Gene Name | MEP |
| Organism | Pleurotus ostreatus (Oyster mushroom) (White-rot fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Pleurotaceae Pleurotus Pleurotus ostreatus (Oyster mushroom) (White-rot fungus) |
| Enzyme Sequence | ATFVGCSATRQTQLNAAASQAQTYAANALSYLNSHTSSTTRYTTWFGTFVTSRYNTVLSHFSSISSNTFSSYTFDCTCSDSGTYAFVNPSNFGYVTLCGAFWNAPVAGTDSRGGTLIHESSHFTRNGGTDDHVYGQAGAQSLARSNPAQAIDNADSHEYFAENNPALA |
| Enzyme Length | 168 |
| Uniprot Accession Number | P81055 |
| Absorption | |
| Active Site | ACT_SITE 119; /evidence=ECO:0000250|UniProtKB:P81054 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by chelating agents such as EDTA and 1,10-phenanthroline. {ECO:0000269|PubMed:8541645, ECO:0000269|Ref.4}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage in proteins: -Xaa-|-Lys- (in which Xaa may be Pro).; EC=3.4.24.20; |
| DNA Binding | |
| EC Number | 3.4.24.20 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Metal binding (3); Site (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Note=Binds strongly to beta-1,3-glucan and chitin, major polysaccharides constituting the fungal cell wall. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 17,925 |
| Kinetics | |
| Metal Binding | METAL 118; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P81054; METAL 122; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P81054; METAL 131; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P81054 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.20; |