| IED ID | IndEnz0002013969 |
| Enzyme Type ID | protease013969 |
| Protein Name |
Alpha-2-macroglobulin Alpha-2-M C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 |
| Gene Name | A2M CPAMD5 FWP007 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA |
| Enzyme Length | 1474 |
| Uniprot Accession Number | P01023 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (18); Chain (1); Cross-link (3); Disulfide bond (13); Erroneous initiation (2); Glycosylation (8); Helix (3); Natural variant (5); Region (4); Sequence conflict (8); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Bait region;Direct protein sequencing;Disulfide bond;Glycoprotein;Isopeptide bond;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal;Thioester bond |
| Interact With | Q6PCB6; P63010-2; Q9UII2; Q8WUW1; Q92478; Q9UNS2; P04141; P35222; Q7L576; Q9NR90-2; Q9UHI6; Q9H147; O75616; Q9Y261-2; Q6PIV2; Q9ULV1; P0C0S5; P68431; P09017; Q6DN90-2; Q92993; Q92993-2; P60409; Q8IUC2; Q9UPM6; Q8TBB1; P07948; Q8TDB4; A4FUJ8; P41218; Q9Y605; Q96FW1; Q6GQQ9-2; Q99497; O75925; P27986-2; O75626-3; P57729; Q9ULX5; Q96D59; P04271; Q16637-3; Q7Z6I5; Q9UNE7; O75558; Q8N4C7; P43405-2; P28347-2; Q86WT6-2; Q8WVJ9; P10599; Q9BVJ6; Q9UBQ0-2; Q96AX1; Q8N895; Q99KR7 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:6203908}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000269|PubMed:6203908 |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (3) |
| Cross Reference PDB | 1BV8; 2P9R; 4ACQ; 6TAV; |
| Mapped Pubmed ID | 10203250; 10318830; 10449138; 10462112; 10477119; 10477120; 10527839; 10548657; 10668708; 10668709; 10668711; 10817585; 10936700; 10943700; 10976654; 11036822; 11041282; 11058789; 11099722; 11121179; 11166925; 11231028; 11281447; 11290389; 11436125; 11455130; 11484172; 11496365; 11498265; 11793025; 11811950; 11823454; 11901360; 11910179; 11987698; 12015318; 12042276; 12059070; 12062545; 12123860; 12175343; 12221172; 12394648; 12631277; 12755614; 12755687; 12782337; 12782964; 12966032; 14506912; 14637088; 14675603; 14678766; 14715656; 14759632; 15023809; 15468911; 15511627; 15793779; 15908432; 15910735; 15931081; 16040006; 16169070; 16232083; 16297527; 16384981; 16402284; 16407218; 16456795; 16548525; 16641085; 16650578; 16784755; 16910174; 1698775; 17071617; 17174955; 1724156; 17288987; 17403113; 17580958; 17967834; 18075673; 18077315; 18171086; 18191876; 18382889; 18485748; 18499670; 18579803; 18624398; 18706476; 19074141; 19105203; 19472074; 19639019; 19913121; 20048078; 20085233; 20156641; 20207732; 20217867; 20381197; 20401725; 20493925; 20575108; 20628086; 20637261; 20711500; 20800603; 20861575; 21163940; 21216953; 21541797; 21642630; 21900206; 21971609; 21988832; 22290936; 22541546; 22576683; 22971340; 23064953; 23086799; 23089609; 23186781; 23300094; 23353684; 23379699; 23397525; 23535567; 23602568; 23640497; 23643808; 23721263; 23786280; 23846698; 24093110; 2415929; 24516643; 2467766; 24756728; 24799681; 25241761; 25495992; 25574746; 2579948; 25809189; 25867373; 26134405; 26217017; 26476541; 27002159; 27044074; 27228572; 27498393; 28087279; 28215695; 28253193; 28705706; 28887505; 29093276; 29659051; 30479021; 30564965; 30767554; 31243924; 31506491; 31995266; 32163666; 32541810; 32559179; 32915361; 32957873; 33643290; 6457647; 7559647; 8467233; 9697696; 9811940; |
| Motif | |
| Gene Encoded By | |
| Mass | 163,291 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |