| IED ID | IndEnz0002013970 |
| Enzyme Type ID | protease013970 |
| Protein Name |
Acylamino-acid-releasing enzyme AARE EC 3.4.19.1 Oxidized protein hydrolase OPH |
| Gene Name | AARE At4g14570 dl3325w |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDSSGTDSAKELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSGSQAMFALSQANLLANKKKKFMLSGHISNESNQSVNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNPENESPTKFEIWNSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKPTFDHLGYYKKENSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIPRSISVGQVVWSPNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIKFTSDEPKDDDANEFPIHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWPSDGKLPESTNIVDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILSVNLLSGEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLWSNIQSPIRYSEKVMAGLSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGKCDPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQDVKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMASMVGITDIPDWCFFEAYGDQSHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKDLRVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDRPQTDYESFLNIAVWFNKYCKL |
| Enzyme Length | 764 |
| Uniprot Accession Number | Q84LM4 |
| Absorption | |
| Active Site | ACT_SITE 618; /note="Charge relay system"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10084, ECO:0000269|PubMed:12966075"; ACT_SITE 707; /note="Charge relay system"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10084, ECO:0000269|PubMed:12966075"; ACT_SITE 739; /note="Charge relay system"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10084, ECO:0000269|PubMed:12966075" |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by the serine protease inhibitor diisopropyl fluorophosphate. {ECO:0000269|PubMed:12966075}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.; EC=3.4.19.1; Evidence={ECO:0000269|PubMed:12966075}; |
| DNA Binding | |
| EC Number | 3.4.19.1 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. Can degrade the glycated RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) protein but not the native protein. May be involved in the elimination of glycated proteins (PubMed:12966075). Plays a homeostatic role in sustaining the cytoplasmic antioxidative system. May contribute to the elimination of the oxidized proteins in the cytoplasm (PubMed:22398639). {ECO:0000269|PubMed:12966075, ECO:0000269|PubMed:22398639}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:12966075}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous gene model prediction (2); Mutagenesis (3) |
| Keywords | Cytoplasm;Hydrolase;Nucleus;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22398639}. Nucleus {ECO:0000269|PubMed:22398639}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15215502; 16895613; 18315867; 18431481; 21166475; 27247031; 28627464; |
| Motif | |
| Gene Encoded By | |
| Mass | 83,938 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.5 mM for Ac-Ala-pNA {ECO:0000269|PubMed:12966075}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.19.1; |