| IED ID | IndEnz0002013975 |
| Enzyme Type ID | protease013975 |
| Protein Name |
Truncated polyprotein 1aTF Cleaved into: Nsp1 EC 3.4.22.- ; Nsp1-alpha papain-like cysteine proteinase EC 3.4.22.- PCP1-alpha ; Nsp1-beta papain-like cysteine proteinase EC 3.4.22.- PCP1-beta ; Nsp2TF |
| Gene Name | |
| Organism | Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Arnidovirineae Arteriviridae unclassified Arteriviridae Porcine reproductive and respiratory syndrome virus (PRRSV) Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV) |
| Enzyme Sequence | MSGTFSRCMCTPAARVFWNAGQVFCTRCLSARPLLSPELQDTDLGVVGLFYKPKDKIHWKVPIGIPQVECTPSGCCWLSAVFPLARMTSGNHNFLQRLVKVADVLYRDGCLAPRHLRELQVYERGCSWYPITGPVPGMGLFANSMHVSDQPFPGATHVLTNSPLPQRACRQPFCPFEEAHSDVYRWKKFVIFTDSSPNGRFRMMWTPESDDSAALEVLPPELERQVEILTRSFPAHHPINLADWELTESPENGFSFGTSHSCGHIVQNPNVFDGKCWLTCFLGQSAEVCYHEEHLANALGYQTKWGVHGKYLQRRLQVRGMRAVVDPDGPIHVEALSCSQSWVRHLTLNNDVTPGFVRLTSIRIVSNTEPTAFRIFRFGAHKWYGAAGKRARAKRATKSGKDSALAPKIAPPVPTCGITTYSPPTDGSCGWHVLAAIVNRMINGDFTSPLPQYNRPEDDWASDYDLAQAIQCLQLPATVVRNRACPNAKYLIKLNGVHWEVEVRSGMAPRSLSRECVVGVCSEGCVAPPYPADGLPKRALEALASAYRLPSDCVSSGIADFLADPPPQEFWTLDKMLTSPSPERSGFSSLYKLLLEVVPQKCGATEGAFVYAVERMLKDCPSPEQAMALLAKIKVPSSKAPSVSLDECFPAGVPADFEPAFQERPRSPGAAVALCSPDAKGFEGTASEEAQESGHKAVHAVPLAEGPNNEQVQVVAGEQLELGGCGLAIGSAQSSSDSKRENMHNSREDEPLDLSHPAPAATTTLVGEQTPDNPGSDASALPIAVRGFVPTGPILRHVEHCGTESGDSSSPLDLSFAQTLDQPLDLSLAAWPVKATASDPGWVRGRCEPVFLKPRKAFSDGDSALQFGELSESSSVIEFDQTKDTLVADAPVDLTTSNEALSAVDPSEFVELRRPRHSAQALIDRGGPLADVHAKIKNRVYEQCLQACEPGSRATPATREWLDKMWDRVDMKTWRCTSQFQAGRILASLKFLPDMIQDTPPPVPRKNRASDNAGLKQLVARWDKKLSVTPPPKSAGLVLDQTVPPPTDIQQEDATPSDGLSHASDFSSRVSTSWSWKGLMLSGTRLAGSAGQRLMTWVFLKFTPISQLLYSHFSRRGALWLQAIGCLQVLFYLLSCSVVLTQYSDAFPYWVSFLVLCGVFVWVFLVLGWLLLYFYSRLHPTQSVLLVTTIRRNVMLSFWLLSSANFGNLCAALWLAPQVSYVSSLASYSVGHVISGMLSYVYACLQIWPFLLFMWCPKGVVTSVGESV |
| Enzyme Length | 1268 |
| Uniprot Accession Number | P0DJY0 |
| Absorption | |
| Active Site | ACT_SITE 76; /note=For Nsp1-alpha papain-like cysteine proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00872; ACT_SITE 146; /note=For Nsp1-alpha papain-like cysteine proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00872; ACT_SITE 276; /note=For Nsp1-beta papain-like cysteine proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00873; ACT_SITE 345; /note=For Nsp1-beta papain-like cysteine proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00873; ACT_SITE 429; /note=For Nsp2 cysteine proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00871; ACT_SITE 498; /note=For Nsp2 cysteine proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00871 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.-; 3.4.22.-; 3.4.22.- |
| Enzyme Function | FUNCTION: [Nsp1]: Is essential for viral subgenomic mRNA synthesis. {ECO:0000250}.; FUNCTION: [Nsp1-alpha papain-like cysteine proteinase]: Inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways (By similarity). {ECO:0000250}.; FUNCTION: Nsp1-beta transactivates the programmed ribosomal frameshifting event leading to the expression of the 1aTF polyprotein. {ECO:0000269|PubMed:24825891}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (6); Chain (5); Compositional bias (2); Domain (3); Region (4); Site (2); Transmembrane (4); Zinc finger (1) |
| Keywords | ATP-binding;Helicase;Host cytoplasm;Host endoplasmic reticulum;Host membrane;Host nucleus;Host-virus interaction;Hydrolase;Membrane;Metal-binding;Nucleotide-binding;Protease;Ribosomal frameshifting;Thiol protease;Transmembrane;Transmembrane helix;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Nsp1]: Host nucleus {ECO:0000250}. Host cytoplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Nsp1-alpha papain-like cysteine proteinase]: Host nucleus {ECO:0000250}. Host cytoplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Nsp1-beta papain-like cysteine proteinase]: Host cytoplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Nsp2TF]: Host endoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 139,092 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |