| IED ID | IndEnz0002014012 |
| Enzyme Type ID | protease014012 |
| Protein Name |
Glycyl-glycine endopeptidase ALE-1 EC 3.4.24.75 Staphylolytic enzyme ALE-1 |
| Gene Name | |
| Organism | Staphylococcus capitis |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus capitis |
| Enzyme Sequence | MDTNRKFTLVKSLSIGLGTFLVGSVFLTVNDEASASTKVDAPKVEQEAPAKADAPKVEQEAPAKADAPKVEQEAPAKVDAPKVEQEAPAKVDAPKVEQEAPAKADAPKVEQKRTFVREAAQSNHSASWLNNYKKGYGYGPYPLGINGGNHYGVDFFMNVGTPVRAISDGKIVEAGWTNYGGGNEIGLVENDGVHRQWYMHLSKFNVKVGDRVKAGQIIGWSGSTGYSTAPHLHFQRMTNSFSNNTAQDPMPFLKSAGYGSNSTSSSNNNGYKTNKYGTLYKSESASFTANTDIITRLTGPFRSMPQSGVLRKGLTIKYDEVMKQDGHVWVGYNTNSGKRVYLPVRTWNESTGELGPLWGTIK |
| Enzyme Length | 362 |
| Uniprot Accession Number | O05156 |
| Absorption | |
| Active Site | ACT_SITE 231; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.; EC=3.4.24.75; |
| DNA Binding | |
| EC Number | 3.4.24.75 |
| Enzyme Function | FUNCTION: Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-9.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (7); Chain (1); Compositional bias (1); Domain (1); Metal binding (3); Region (1); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Cell wall biogenesis/degradation;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..35 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1R77; |
| Mapped Pubmed ID | 16257954; |
| Motif | |
| Gene Encoded By | |
| Mass | 39,350 |
| Kinetics | |
| Metal Binding | METAL 150; /note=Zinc; /evidence=ECO:0000305; METAL 154; /note=Zinc; /evidence=ECO:0000305; METAL 233; /note=Zinc; /evidence=ECO:0000305 |
| Rhea ID | |
| Cross Reference Brenda |