| IED ID | IndEnz0002014036 |
| Enzyme Type ID | protease014036 |
| Protein Name |
Carboxypeptidase B EC 3.4.17.2 47 kDa zymogen granule membrane-associated protein ZAP47 |
| Gene Name | CPB1 CPB |
| Organism | Canis lupus familiaris (Dog) (Canis familiaris) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Carnivora Caniformia Canidae (dog coyote wolf fox) Canis Canis lupus (Gray wolf) Canis lupus familiaris (Dog) (Canis familiaris) |
| Enzyme Sequence | MAFLILVTLALASAHYSGEHFEGEKVFRVNVEDENHINLLHTLASTTQIDFWKPDSVTQIKPHSTADFRVKAEDILTVEDFLKQNELHYEVLINNLRLVLEGQFGRQVPATGHSYEKYNRWETIEAWTQQVTSENPDLISRRSIGTTFEGRTIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFWQWFVREXIRTYGQEIHMTELLDKLDFYVLPVGNIDGYVYTWTKNRMWRKTRSTQVGTNCVGTDPTRNFDAGWCKIGASRNPCDETYCGPAAESEKETKALANFIRSNLSSIKAYLTIHSYSQMMLYPYSYDYKLTENNAELNALAKATVKELATLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIKYSFTFELRDKGRYGFALPESQISPTCEETLLAIKHLARYVLQHLY |
| Enzyme Length | 416 |
| Uniprot Accession Number | P55261 |
| Absorption | |
| Active Site | ACT_SITE 377; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P00732 |
| Activity Regulation | |
| Binding Site | BINDING 233; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00730; BINDING 355; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00730 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential release of a C-terminal lysine or arginine amino acid.; EC=3.4.17.2; Evidence={ECO:0000250|UniProtKB:P00732}; |
| DNA Binding | |
| EC Number | 3.4.17.2 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Disulfide bond (2); Metal binding (3); Propeptide (1); Region (3); Signal peptide (1) |
| Keywords | Carboxypeptidase;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:7765250}. Zymogen granule lumen {ECO:0000305|PubMed:7765250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..15; /evidence=ECO:0000250|UniProtKB:P15086 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,597 |
| Kinetics | |
| Metal Binding | METAL 175; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086; METAL 178; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086; METAL 303; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086 |
| Rhea ID | |
| Cross Reference Brenda |