| IED ID | IndEnz0002014038 |
| Enzyme Type ID | protease014038 |
| Protein Name |
Cathepsin B-like protease 1 EC 3.4.22.- Cathepsin B1 AtCathB1 |
| Gene Name | CATHB1 At1g02300 T7I23.12 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MADSCCIRLHLLASVFLLLFSSFNLQGIAAENLSKQKLTSLILQNEIVKEVNENPNAGWKAAFNDRFANATVAEFKRLLGVIQTPKTAYLGVPIVRHDLSLKLPKEFDARTAWSHCTSIRRILVGYILNNVLLWSTITLWFWFLLGHCGSCWAFGAVESLSDRFCIKYNLNVSLSANDVIACCGLLCGFGCNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEQSVVAGLPSEKNVFKGITTSDDLLVSSV |
| Enzyme Length | 379 |
| Uniprot Accession Number | F4HVZ1 |
| Absorption | |
| Active Site | ACT_SITE 151; /evidence=ECO:0000250|UniProtKB:P07858; ACT_SITE 306; /evidence=ECO:0000255|PROSITE-ProRule:PRU10089; ACT_SITE 327; /evidence=ECO:0000250|UniProtKB:P07858 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Thiol protease that plays a central role in plant programmed cell death (PCD). In addition to its role in protein degradation, may cleave and/or degrade a number of target proteins, activating signaling towards PCD. Contributes to the increase of caspase-3-like activity after UV-C-induced PCD and is required for abiotic stress-induced PCD (PubMed:27058316). Functions redundantly with CATHB2 and CATHB3 in basal defense and distinct forms of plant programmed cell death (PCD). Participates in the establishment of basal resistance against the bacterial pathogen Pseudomonase syringae pv. tomato DC3000. Required for full levels of PCD during resistance (R) gene-mediated hypersensitive response (HR). Involved in the regulation of senescence, a developmental form of PCD in plants (PubMed:19453434). {ECO:0000269|PubMed:19453434, ECO:0000269|PubMed:27058316}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (6); Erroneous gene model prediction (1); Glycosylation (4); Propeptide (2); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Plant defense;Protease;Reference proteome;Signal;Thiol protease |
| Interact With | |
| Induction | INDUCTION: By dark-induced senescence. Down-regulated by infection with an avirulent strain of the bacterial pathogen Pseudomonase syringae pv. tomato DC3000. {ECO:0000269|PubMed:19453434}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..30; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14617064; 14697270; 15047901; 16911220; 17259181; 18650403; 20736450; 28675441; 29924726; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,263 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |