| IED ID | IndEnz0002014042 |
| Enzyme Type ID | protease014042 |
| Protein Name |
Carboxypeptidase B EC 3.4.17.2 |
| Gene Name | Cpb1 Cpb |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MLLLLALVSVALAHASEEHFDGNRVYRVSVHGEDHVNLIQELANTKEIDFWKPDSATQVKPLTTVDFHVKAEDVADVENFLEENEVHYEVLISNVRNALESQFDSHTRASGHSYTKYNKWETIEAWIQQVATDNPDLVTQSVIGTTFEGRNMYVLKIGKTRPNKPAIFIDCGFHAREWISPAFCQWFVREAVRTYNQEIHMKQLLDELDFYVLPVVNIDGYVYTWTKDRMWRKTRSTMAGSSCLGVRPNRNFNAGWCEVGASRSPCSETYCGPAPESEKETKALADFIRNNLSTIKAYLTIHSYSQMMLYPYSYDYKLPENYEELNALVKGAAKELATLHGTKYTYGPGATTIYPAAGGSDDWSYDQGIKYSFTFELRDTGFFGFLLPESQIRQTCEETMLAVKYIANYVREHLY |
| Enzyme Length | 415 |
| Uniprot Accession Number | P19223 |
| Absorption | |
| Active Site | ACT_SITE 376; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P00732 |
| Activity Regulation | |
| Binding Site | BINDING 232; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00730; BINDING 354; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00730 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential release of a C-terminal lysine or arginine amino acid.; EC=3.4.17.2; Evidence={ECO:0000250|UniProtKB:P00732}; |
| DNA Binding | |
| EC Number | 3.4.17.2 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Disulfide bond (3); Metal binding (3); Propeptide (1); Region (3); Signal peptide (1) |
| Keywords | Carboxypeptidase;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P00732}. Zymogen granule lumen {ECO:0000250|UniProtKB:P55261}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..13; /evidence=ECO:0000250|UniProtKB:P15086 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14977629; 16522183; 17366889; 17906107; 22112400; 28949379; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,515 |
| Kinetics | |
| Metal Binding | METAL 174; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086; METAL 177; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086; METAL 302; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086 |
| Rhea ID | |
| Cross Reference Brenda |