Detail Information for IndEnz0002014049
IED ID IndEnz0002014049
Enzyme Type ID protease014049
Protein Name Carboxypeptidase B2
EC 3.4.17.20
Carboxypeptidase R
CPR
Carboxypeptidase U
CPU
Thrombin-activable fibrinolysis inhibitor
TAFI
Gene Name Cpb2 Tafi
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MKLHGLGILVAIILYEQHGFAFQSGQVLSALPRTSRQVQLLQNLTTTYEVVLWQPVTAEFIEKKKEVHFFVNASDVDSVKAHLNVSRIPFNVLMNNVEDLIEQQTFNDTVSPRASASYYEQYHSLNEIYSWIEVITEQHPDMLQKIYIGSSFEKYPLYVLKVSGKEQRIKNAIWIDCGIHAREWISPAFCLWFIGYVTQFHGKENLYTRLLRHVDFYIMPVMNVDGYDYTWKKNRMWRKNRSAHKNNRCVGTDLNRNFASKHWCEKGASSSSCSETYCGLYPESEPEVKAVADFLRRNIDHIKAYISMHSYSQQILFPYSYNRSKSKDHEELSLVASEAVRAIESINKNTRYTHGSGSESLYLAPGGSDDWIYDLGIKYSFTIELRDTGRYGFLLPERYIKPTCAEALAAISKIVWHVIRNT
Enzyme Length 422
Uniprot Accession Number Q9JHH6
Absorption
Active Site ACT_SITE 384; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P00732
Activity Regulation ACTIVITY REGULATION: TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-323 (By similarity). {ECO:0000250}.
Binding Site BINDING 238; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00730; BINDING 362; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00730
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of C-terminal Arg and Lys from a polypeptide.; EC=3.4.17.20;
DNA Binding
EC Number 3.4.17.20
Enzyme Function FUNCTION: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Binding site (2); Chain (1); Disulfide bond (3); Glycosylation (6); Metal binding (3); Propeptide (1); Region (3); Sequence conflict (3); Signal peptide (1); Site (1)
Keywords Blood coagulation;Carboxypeptidase;Disulfide bond;Fibrinolysis;Glycoprotein;Hemostasis;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10739389}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11154109; 11217851; 11781355; 12417566; 12466851; 12645517; 14521589; 14610273; 14681479; 15099284; 15383602; 15647842; 16272333; 16565485; 17105819; 17111204; 17988229; 18554416; 18612543; 19501082; 20088932; 20431069; 20657835; 20723031; 21395696; 21491542; 21724997; 21804193; 22104553; 22217421; 23467679; 23487423; 23721130; 24449579; 25401979; 25851247; 26324704; 26340319; 26611327; 28289017; 30026184; 31465300; 32506822;
Motif
Gene Encoded By
Mass 48,871
Kinetics
Metal Binding METAL 180; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086; METAL 183; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086; METAL 309; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P15086
Rhea ID
Cross Reference Brenda 3.4.17.20;