| IED ID | IndEnz0002014064 |
| Enzyme Type ID | protease014064 |
| Protein Name |
Disintegrin and metalloproteinase domain-containing protein 25 ADAM 25 EC 3.4.24.- Testase-2 |
| Gene Name | Adam25 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MQTTQRASSFAAAEDNIAMDKAVVYTRIPHLYLWLEILNILSSRPLTGYAQHTSLPEVVIPLRVTGNRPMWAMGWLTYSLHFGGQKHFIHIKAKKFLVSRLFSVFTYTKQGALHKDQPYVQNDCYYHGHMDGDPESMVAITTCYGGFQGILQINGTVYEIKPKNLSSTFEHLVHKMDSEETELLPMRCALTEEIARQMKLQQNENPTLMQSHYEGWWTHKSFLDLALVVERERIRYHNNNTSRVLVEVFTIINIINNIYETLDVELVLLGVEMWNERNHVQVRSIEELLDEFCMWKARSLNFRIPNDIAHIFVNHEFGIYLGLAYIGSVCVPSHNCGVDRLLGGNLFYFGRIIAHEMGHNLGMEHDSSSCTCGTKICLMAPADNGIPKFSNCSYSYYWATYATAKCMRKEKKSKGILRGKLCGDGVVDDGEQCDCGSAKSCADDPCCKPSCTLKDGAACAFGLCCLYCQIMPAGTVCRQEVNECDLPEWCNGHSHKCPNDVYLLDGSPCRDGGYCYEKRCNNRDEQCKQIFGKEARSADHSCYRELNTQGDRFGNCGVIRDAYLRCHDPDILCGRVQCENVAHIPFLRDHSTVHWTHLNGVTCWGTDYHFGMTIPDIGIVKDGTDCGPEHVCINKKCVSKSIWRSQCSPKTCNMKGVCNNLHHCHCNLGWDPPHCLKSGLGGSIDSGPPNYTENYTEKKHKKSIGLVILFWILFACFSVLFIVFLFFLRSYVELPMSEEPKVPTPENKEDTNEVMNTETE |
| Enzyme Length | 760 |
| Uniprot Accession Number | Q9R159 |
| Absorption | |
| Active Site | ACT_SITE 356; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Disulfide bond (7); Domain (3); Glycosylation (7); Metal binding (4); Motif (1); Propeptide (1); Region (1); Sequence conflict (6); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Developmental protein;Differentiation;Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Spermatogenesis;Transmembrane;Transmembrane helix;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..50; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 21267068; |
| Motif | MOTIF 186..193; /note=Cysteine switch; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 86,128 |
| Kinetics | |
| Metal Binding | METAL 188; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 355; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 359; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 365; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |