| IED ID | IndEnz0002014081 |
| Enzyme Type ID | protease014081 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA Rsph17025_1122 |
| Organism | Cereibacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) (Rhodobacter sphaeroides) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Rhodobacterales Rhodobacteraceae Cereibacter Cereibacter sphaeroides (Rhodobacter sphaeroides) Cereibacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) (Rhodobacter sphaeroides) |
| Enzyme Sequence | MTHPVPIQFQSPDLEAIASSAGRIVVFAGEGGAMGVAAKRVNRLMRGALERAAASEAFGKLKQGEAMELGFPAGMAADAVQLVKLDRRCDVATARKAGGAIGRSLSKAGTLVLADTIQRAAEVSFGIALRAYDFTPHKTGEKTVPGPVTMMVANPETVAAEAGPMAALAEGIFFTRDLVNEPANVLSTFDFAARLAAMHELGLEVEILEEEDMEKLGMRALLGVGQGSEHPSKLVVMQWKGGPEDQVPLALVGKGVVFDTGGISIKPAAGMEDMTMDMGGAAVVAGVMRTLAMRKARANVVGLVGIVENMPDGNAQRPGDVVRSMKGDTIEVINTDAEGRLVLADVLWYAQERFNPRGVIDLATLTGAIIVALGHENTGVFSNDDAFCAAFLKAAQSEGEGAWRMPMGERYDEMLKSRIADMRNSTGREAGSITAAHFLRRFVKPETPWIHLDIAGTALLKGDTALAPKGATGWGVLSLDRLIRDMLEK |
| Enzyme Length | 489 |
| Uniprot Accession Number | A4WRK9 |
| Absorption | |
| Active Site | ACT_SITE 266; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 340; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,765 |
| Kinetics | |
| Metal Binding | METAL 254; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 259; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 259; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 277; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 336; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 338; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 338; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |