| IED ID | IndEnz0002014087 |
| Enzyme Type ID | protease014087 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA CPE2501 |
| Organism | Clostridium perfringens (strain 13 / Type A) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium perfringens Clostridium perfringens (strain 13 / Type A) |
| Enzyme Sequence | MYNFLVKKSKCTETDALLIPYFEEKTNIPNEEINNKINILKKKEQFKGSYGEIFNITRTTDDNIQDIILLGLGKETEITKEKIRRAFGKAVNEIKRLKSKSVFLRFDSVEAIGLENTLKAMVEGLALGSYSFNKYKSDKKEEVEVTVHIGGHNINEEEVELCEKAIDEAMLLSETTCLARDLVNEPANNMYPETLAKEVKNIGSKYGFEVEVFDENQIEELKMESFLSVGKGSDNLPRLIVMRYFGDKDNMDQRLALVGKGLTYDSGGYSLKTNAGMVTMKADMGGAASVIGAISAIAKRNLKINVIAVVAACENLISGHAYKPGDIIGSMEGKTIEILNTDAEGRLTLIDAVTYAIEKEKASEIIDVATLTGAAVVSLGEDVTAVITNKDEFYGELREASYETGEKVWQMPSFEDYGKLIKSNIADLKNIGGKYAGTITAGLFIGEFIQNKPWLHLDIAGPAFSEKKGDYCPTGGTGAGVRTLYELANRRCK |
| Enzyme Length | 493 |
| Uniprot Accession Number | Q8XHI3 |
| Absorption | |
| Active Site | ACT_SITE 272; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 346; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,307 |
| Kinetics | |
| Metal Binding | METAL 260; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 265; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 265; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 283; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 342; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 344; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 344; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |