| IED ID | IndEnz0002014180 |
| Enzyme Type ID | protease014180 |
| Protein Name |
Apoptotic protease-activating factor 1 APAF-1 |
| Gene Name | APAF1 KIAA0413 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE |
| Enzyme Length | 1248 |
| Uniprot Accession Number | O14727 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 265; /note=ATP; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {ECO:0000269|PubMed:10393175, ECO:0000269|PubMed:12804598}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 154..161; /note=ATP; /evidence=ECO:0000255 |
| Features | Alternative sequence (6); Beta strand (8); Binding site (1); Chain (1); Domain (2); Frameshift (1); Helix (39); Mutagenesis (2); Nucleotide binding (1); Region (1); Repeat (15); Sequence conflict (23); Turn (4) |
| Keywords | 3D-structure;ATP-binding;Alternative splicing;Apoptosis;Calcium;Cytoplasm;Direct protein sequencing;Nucleotide-binding;Reference proteome;Repeat;WD repeat |
| Interact With | P55211; P99999; P62136; P62258 |
| Induction | INDUCTION: By E2F and p53/TP53 in apoptotic neurons (PubMed:11389439). Translation is inhibited by HNRPA1, which binds to the IRES of APAF1 mRNAs (PubMed:31498791). {ECO:0000269|PubMed:11389439, ECO:0000269|PubMed:31498791}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12804598}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (2); Electron microscopy (4); X-ray crystallography (7) |
| Cross Reference PDB | 1C15; 1CWW; 1CY5; 1Z6T; 2P1H; 2YGS; 3J2T; 3JBT; 3YGS; 4RHW; 5JUY; 5WVC; 5WVE; |
| Mapped Pubmed ID | 10206961; 10376594; 10500165; 10929711; 10934467; 10949025; 11084335; 11257231; 11550094; 11559530; 11701129; 11734640; 11752425; 11864614; 12021264; 12149244; 12615903; 12620238; 12637514; 12824163; 12963020; 14566819; 14747474; 14960576; 14993223; 15009102; 15026369; 15033720; 15271982; 15305193; 15378005; 15590702; 15649154; 15692060; 15703181; 15829969; 15832174; 15832175; 15863166; 15972851; 16046141; 16098052; 16231040; 16232302; 16331630; 16595687; 16916640; 16951219; 17133271; 17274640; 17348858; 17361096; 17408690; 17534194; 17541304; 17600109; 17603079; 17876870; 17882496; 17885667; 18042457; 18083711; 18093951; 18373966; 18439902; 18587251; 18619610; 18676680; 18719738; 18834886; 19058789; 19074885; 19141860; 19170196; 19238172; 19455599; 19494828; 19603830; 19625176; 19662370; 19692168; 19801675; 19809088; 19913121; 20216471; 20345447; 20379614; 20453000; 20462491; 20628086; 20959405; 20977544; 21104989; 21297999; 21528061; 21536655; 21659556; 21827945; 21922274; 22101335; 22246185; 22335196; 22996741; 23085065; 23207240; 23326121; 23521171; 23650375; 23650620; 23822711; 24038028; 24367089; 24416260; 24424093; 25313070; 25330150; 25599959; 25701323; 25895636; 26014357; 26179084; 26183023; 26290316; 26543158; 26740177; 26743285; 26851285; 27021436; 27032384; 27141571; 27480415; 27697150; 27863378; 28111022; 28143931; 28192157; 28423356; 28429233; 28627586; 28895780; 28982084; 29352212; 29358613; 29395479; 29410086; 29511177; 30066861; 30072015; 31094091; 31131537; 31255735; 31329620; 32190895; 32461338; 32653692; 33132160; 33177505; 33308446; 33386492; 34507064; 9230442; 9390557; 9829980; 9922454; |
| Motif | |
| Gene Encoded By | |
| Mass | 141,840 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |