| IED ID | IndEnz0002014199 |
| Enzyme Type ID | protease014199 |
| Protein Name |
Leucine aminopeptidase 2, chloroplastic EC 3.4.11.1 Leucyl aminopeptidase 2 LAP 2 Proline aminopeptidase 2 EC 3.4.11.5 Prolyl aminopeptidase 2 |
| Gene Name | LAPA2 |
| Organism | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
| Enzyme Sequence | MNGVLCSSSSSFHSYPSIFTKFQSSPIWSFSISVTPLCSRRAKRMAHSIARDTLGLTHTNQSDAPKISFAAKEIDLVEWKGDILTVGATEKDLARDGNSKFQNPLLQKLDSKLSGLLSEASSEEDFSGKAGQSTILRLPGLGSKRIALVGLGSPTSSTAAYRCLGEAAAAAAKSAQASNIAIALASTDGLSAELKLSSASAITTGAVLGTFEDNRFKSESKKPTLKSLDILGLGTGPEIEKKIKYAADVCAGVILGRELVNAPANVLTPAVLAEEAKKIASTYSDVFSANILDVEQCKELKMGSYLRVAAASANPAHFIHLCYKPSSGEIKKKIALVGKGLTFDSGGYNIKTGPGCSIELMKFDMGGAAAVLGAAKALGQIKPAGVEVHFIVAACENMISGTGMRPGDIITASNGKTIEVNNTDAEGRLTLSVGISCNQGVEKIVDLATLTGACVVALGPSIAGIFTPSDDLAKEVVAASEVSGEKLWRLPMEDSYWDSMKSGVADMVNTGGRPGGAITAALFLKQFVNEKVQWMHIDLAGPVWSDKKKNATGFGVSTLVEWVLKNSTN |
| Enzyme Length | 569 |
| Uniprot Accession Number | Q42876 |
| Absorption | |
| Active Site | ACT_SITE 351; /evidence=ECO:0000255; ACT_SITE 428; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.5 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7); Transit peptide (1) |
| Keywords | Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Plastid;Protease;Reference proteome;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By wounding. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17896114; 19376935; |
| Motif | |
| Gene Encoded By | |
| Mass | 59,549 |
| Kinetics | |
| Metal Binding | METAL 339; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 344; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 344; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 364; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 424; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 426; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 426; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184 |
| Rhea ID | |
| Cross Reference Brenda |