| IED ID | IndEnz0002014256 |
| Enzyme Type ID | protease014256 |
| Protein Name |
Calpain-type cysteine protease ADL1 EC 3.4.22.- Phytocalpain ADL1 Protein ADAXIALIZED LEAF1 Protein DEFECTIVE KERNEL 1 OsDEK1 Protein SHOOTLESS 3 |
| Gene Name | ADL1 DEK1 ODM63 SHL3 Os02g0709400 LOC_Os02g47970 OJ1311_H06.4 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MEEEEHRGVVLVCSICGFLFAVLGPLSFWILWAVNWRPWRLYSWIYARKWPAYVQGPQLSTLCSFFTLFAWLVVVSPITVLLVWGGILIALLERNIIGLAVIMVGVALLLSFYSIMLWWRTQWQSSKAVAYLLLLAVGLLCAYEFCAVYVTTGASASELNSPSGFFFGVSAISLAINMLFISKILFNGSGFDVDEYVRRLYKFAYSDCVEVAPVSCSPDPPDPSELYMTKSSRVLHLGLLYLCSLMVLVVYSILYGLTSKEARWLGALTSVAVVILDWNLGLCSFRFELLKSRMIALFVAGTSRVFLICFGVHYWYLGHCISYAFVASVLLAAAVSCWLSISNPSVARIDALRSTVIKLREGFRRKGQTSSSNSSDGCGSSVKRSSGSVEAGPHGNATDSMYRSNSQSDCVNWNNVPFDRSNSCQEGQSSDKNIDSGRASLAHRSNSCLSAVAVQDPETAVVSADRHGDPTASLVVCSSSGLESQGCESSGSATASGNQQLLDLNLAAIFQDRLNDPRITSMLKRNGGLGDVELANLLQDKGLDPNFSYMMKDKVMDPRILALLQRSSLDADREHQDDVDVTGTDSDRLDTTIANQISLSEELRRSGLENWLNLSRLMFHQVAGSPIRAFVVFTLIFIIETVTVAVHRPKPIKVINATHEQFEFGFSILLLSPVVCSIMAFIWSLCAEEMTMTSKPRKYGFIAWLLSTCVGLLLSFLSKSSVILGLSLTVPLMVACLSFAIPIWMRNGYRFWIPGGELDSRENIRQAPGKKERALFAISITVFTASVIGLGAIVSAKPLDALGYKGWDADKKSFYSPYATSMYLGWALSSTIAVLATGVIPIVAWFATYRFSPSSAICVGLFATVLVSFCGVSYWGVVNSRQDGVPLKADFLAALLPLLCIPAVFSLFTGMYKWKDDDWKISRGVYLFVGMGVLLLLGAISAVIVTIRPWTVGVACLLVILFLVFAIGVIHYWTSNNFYLTRTQMLLVCSLAFLLALAAFLMGLFQEKPFVGASIGYFSFLFLLTGRALTVLLSPPIVVYSPRVLPVYVYDAHADSAKNVSYAFLILYGIALATEVWGVIASLILNPPFIGAAISAITLVIAFSFAVSRPCLTLKMLEDAVHFLSKDTVVQAMSRSANKTRNAISGTYSAPQRSASSAALLVGDPAITLDRAGNFVLPRADVMKLRDRLRNEEITAGSFFCGVKNCLMIGSPVDVDYRRNMCAHARILALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAEQIQDEVRLRLFLDSIGLSDLSAKEIKKWMPEDRRHFELIQESYIREKEMEEEVLMQRREEEGKGRERRKALLEREERKWKELEISLLSSIPNAGSRDAAAMAAAVRAVGGDSALEDSFARDRVSSIARHIRKAQLARRAEQTGIPDTVCILDDEPRSTGRHCGEIDLCLCESKKVSFSIAVMVQPVSGPVCLFGTEFQKKVCWEILVAGSEQGMEAGQVGLRLVTKGERMTTVAKEWNIGASSIADGRWHLVTVTIDADLGEATSFIDGVYDGYQNALPLPRNNGIWEPGTDIWVGARPPTDLDAFGRSDSEGSDSKMQIMDAFLWGRCLTEDEVAMLHTAICSAEYGLFDLAAEDAWHGSYSARVDDWESEEANFELYDQEDVEWDGQYSSGRKRHARDSVAIDIDSFARRPRKPRFETREEVNQRMLSVERAVREALIAKGERNFTDQEFPPDDRSLFVDPMNPSLKLQVVSEWMRPSDIAKEVSISSQPCLFSGSVNSSDVCQGRLGDCWFLSAVAVLTEMARISEVIITPEYNEEGIYTVRFCIQGEWVAVVVDDWIPCESPGKPAFATSRKQNELWVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSPQAQIDLASGRLWSQLLHFKQEGFLLGAGSPSGSDAHISSSGIVQGHAYSILQVREVDGHKLVQIRNPWANEVEWNGPWSDSSQEWTERMKHKLKHVPQSKNGVFWMSWQDFQIHFRSIYVCRVYPPEMRYSVHGQWRGYSAGGCQDYDSWHQNPQYRLRVTGRDALYPVHVFITLTQGVGFSRKTNGFRNYQSSHDSSMFYIGMRILKTRGCRAAYNIYMHESVGGTDYVNSREISCELVLEPYPKGYTIVPTTIHPGEEAPFVLSVFTKAPIKLEAV |
| Enzyme Length | 2162 |
| Uniprot Accession Number | Q6ZFZ4 |
| Absorption | |
| Active Site | ACT_SITE 1772; /evidence=ECO:0000250; ACT_SITE 1930; /evidence=ECO:0000250; ACT_SITE 1950; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Essential protease involved in epiderm development. Required for aleurone cell development in the endosperm probably by maintaining and restricting the aleurone and embryonic epidermal L1 cell-layer fates as well as meristems organization. Involved in the maintenance of adaxial/abaxial axis information in developing leaves, probably by regulating cell proliferation and expansion. Does not need calcium ions to be active. {ECO:0000269|PubMed:10409508, ECO:0000269|PubMed:19665012, ECO:0000269|Ref.6}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (2); Modified residue (3); Mutagenesis (4); Propeptide (1); Region (1); Sequence conflict (2); Signal peptide (1); Topological domain (22); Transmembrane (21) |
| Keywords | Cell membrane;Cytoplasm;Developmental protein;Endoplasmic reticulum;Endosome;Hydrolase;Membrane;Phosphoprotein;Protease;Reference proteome;Repeat;Signal;Thiol protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Endosome membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | MOD_RES 1372; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 1377; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 1668; /note=Phosphoserine; /evidence=ECO:0000250 |
| Post Translational Modification | PTM: Autocatalytic proteolytic cleavage leading to the production of mainly cytoplasmic localized subproducts of about 85 and 120 kDa. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..33; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 239,859 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |