IED Industry Enzyme Database

Detail Information for IndEnz0002014259
IED ID IndEnz0002014259
Enzyme Type ID protease014259
Protein Name Death-associated inhibitor of apoptosis 1
EC 2.3.2.27
Apoptosis 1 inhibitor
E3 ubiquitin-protein ligase th
Inhibitor of apoptosis 1
Protein thread
RING-type E3 ubiquitin transferase Diap1
Gene Name Diap1 Iap1 th CG12284
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS
Enzyme Length 438
Uniprot Accession Number Q24306
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:14517550, ECO:0000269|PubMed:21145488, ECO:0000269|PubMed:22304967};
DNA Binding
EC Number 2.3.2.27
Enzyme Function FUNCTION: Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Dronc, and effector caspases Drice and Dcp-1. Acts as a Nedd8-E3 ubiquitin-protein ligase for Drice. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and hid-dependent cell death in the eye. Interaction of Diap1 with Dronc is required to suppress Dronc-mediated cell death through Diap1-mediated ubiquitination of Dronc. Acts as a positive regulator of Wnt signaling. {ECO:0000269|PubMed:14517550, ECO:0000269|PubMed:17397804, ECO:0000269|PubMed:18259196, ECO:0000269|PubMed:21145488, ECO:0000269|PubMed:22304967, ECO:0000269|PubMed:8548811}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (8); Chain (1); Erroneous translation (1); Helix (13); Metal binding (4); Mutagenesis (2); Region (2); Repeat (2); Sequence conflict (2); Site (2); Turn (4); Zinc finger (1)
Keywords 3D-structure;Apoptosis;Metal-binding;Reference proteome;Repeat;Transferase;Ubl conjugation;Ubl conjugation pathway;Wnt signaling pathway;Zinc;Zinc-finger
Interact With P25843; Q9XYF4; Q24106; Q86PE7; Q24475
Induction
Subcellular Location
Modified Residue
Post Translational Modification PTM: Ubiquitinated and degraded by HtrA2 in apoptotic cells; proteolytic cleavage at specific sites in the BIR domain linker region generating inactive fragments. Mutation of one site reduces but does not abolish cleavage as another site is selected by the protease. {ECO:0000269|PubMed:17397804, ECO:0000269|PubMed:18259196}.
Signal Peptide
Structure 3D X-ray crystallography (8)
Cross Reference PDB 1JD4; 1JD5; 1JD6; 1Q4Q; 1SDZ; 1SE0; 3SIP; 3SIQ;
Mapped Pubmed ID 10077606; 10200472; 10200511; 10407412; 10471706; 10471712; 10481910; 10491396; 10511707; 10559952; 10580984; 10591654; 10593985; 10611682; 10655220; 10675328; 10684245; 10684252; 10704400; 10733523; 10757786; 10764741; 10766844; 10772791; 10825159; 10854336; 10908589; 10950309; 10984473; 11007472; 11042680; 11046149; 11048731; 11102374; 11102824; 11139272; 11139274; 11139276; 11175357; 11178240; 11287192; 11329368; 11337486; 11585906; 11711427; 11717313; 11732848; 11818063; 11818065; 11901172; 11980716; 11997504; 12006672; 12011068; 12021767; 12021768; 12021769; 12021770; 12021771; 12021772; 12042828; 12049762; 12051824; 12062036; 12062163; 12065414; 12070100; 12070612; 12072176; 12110175; 12176339; 12198495; 12198529; 12202030; 12202035; 12202036; 12356728; 12397080; 12427028; 12441239; 12445403; 12446669; 12525502; 12547518; 12663920; 12692559; 12724766; 12729588; 12783792; 12783893; 12807778; 12814564; 12814944; 12844367; 12850443; 12853472; 12885559; 12906812; 12917412; 12930749; 12938178; 12941273; 12941274; 12944419; 12946623; 12967554; 1358056; 14502294; 14502295; 14523394; 14523431; 14526388; 14527345; 14532134; 14561421; 14561774; 14576353; 14597569; 14605208; 14623440; 14644196; 14647296; 14657035; 14668372; 14668378; 14724249; 14737191; 14744432; 14764878; 14980217; 14996492; 15082313; 15084254; 15088666; 15107838; 15150408; 15238527; 15239946; 15242648; 15254017; 15268856; 15337122; 15355784; 15371434; 15383617; 15385155; 15454540; 15464574; 15469838; 15494451; 15501232; 15522457; 15534205; 15573123; 15580265; 15647755; 15704116; 15710747; 15713840; 15744048; 15766530; 15774476; 15776001; 15782175; 15798200; 15800001; 15834148; 15860727; 15972466; 15998717; 1601188; 16096061; 16099837; 16118195; 16170305; 16212942; 16222340; 16243437; 16273080; 16322754; 16341207; 16359857; 16399076; 16404163; 16410550; 16439203; 16456582; 16463014; 16465286; 16485033; 16528381; 16595176; 16611691; 16612543; 16645642; 16672345; 16749923; 16772168; 16797177; 16860746; 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23725764; 23752589; 23784845; 23810935; 23811114; 23846225; 23874212; 23878397; 23911328; 23940367; 23940457; 23943356; 23944235; 23951408; 23955303; 23968895; 23979704; 23999857; 24012335; 24018644; 24050232; 24098761; 24106343; 24137538; 24144920; 24173801; 24184101; 24229347; 24268699; 24291519; 24333635; 24335255; 24339790; 24360962; 24362437; 24374353; 24374974; 24398387; 24509725; 24550005; 24643212; 24658687; 24793180; 24798736; 24819147; 24858703; 24863159; 24892285; 24896182; 24907259; 24907422; 24931167; 24949430; 24981611; 2499514; 24995985; 25020150; 25027438; 25036253; 25097728; 25126784; 25139236; 2515113; 25157415; 25160975; 25188515; 25217223; 25288684; 25299777; 25344916; 25398939; 25412630; 25446534; 25453105; 25511196; 25531226; 25579975; 25634895; 25643297; 25653450; 25668151; 25687947; 25692647; 25719210; 25740230; 25757939; 25762498; 25792727; 25796446; 25798058; 25806519; 25826608; 25836675; 25850671; 25855961; 25868821; 25880195; 25894556; 25941003; 25951229; 25959206; 25963257; 25968311; 26010168; 26021298; 26069889; 26088162; 26092529; 26117838; 26134911; 26143992; 26151599; 26160069; 26173873; 26175940; 26202785; 26207831; 26212135; 26250909; 26287461; 26291171; 26304727; 26305360; 26362609; 26364751; 26374522; 26395494; 26474042; 26500262; 26546607; 26573956; 26659252; 26711898; 26719127; 26853359; 26853366; 26853367; 26884181; 26898471; 26900866; 26914287; 26954546; 26960254; 26972460; 26972874; 26974344; 26996505; 26998322; 27104928; 27124581; 27136547; 27177021; 27184390; 27197757; 27212238; 27256879; 27298020; 27317679; 27322327; 27348811; 27351747; 27358477; 27378074; 27402810; 27404003; 27437768; 27518434; 27527793; 27528688; 27558818; 27584613; 27783936; 27893747; 27979972; 27992405; 27999411; 28030561; 28045022; 28068320; 28076795; 28085885; 28102471; 28143945; 28207763; 28246211; 28265104; 28418397; 28455414; 28524858; 28542149; 28564598; 28620086; 28637711; 28688927; 28697337; 28704652; 28743877; 28751499; 28815537; 28870988; 28893998; 28900135; 28916802; 28935711; 28945745; 29033939; 29050293; 29142303; 29155828; 29207260; 29231806; 29385168; 29397869; 29403026; 29410178; 29420531; 29440430; 29514098; 29514136; 29535423; 29602952; 29693007; 29695716; 29719260; 29719584; 29750169; 29752350; 29765022; 29804808; 29846027; 29854765; 29881950; 29953853; 29964003; 29970915; 30022065; 30092221; 30157423; 30325918; 30328653; 30359736; 30413561; 30467079; 30504772; 30554899; 30590040; 30595382; 30622305; 30637539; 30650359; 30679505; 30696713; 30718556; 30733258; 30741633; 30765784; 30770378; 30833576; 30836847; 30879903; 30901309; 30904193; 30912203; 31000600; 31040111; 31068592; 31112748; 31155359; 31255773; 31315941; 31409797; 31477571; 31511495; 31520355; 31548372; 31549655; 31553691; 31566767; 31568497; 31597093; 31678471; 31722958; 31745213; 31777182; 31795326; 31828974; 31909714; 31917525; 31941789; 32019113; 32037698; 32053595; 32098783; 32117966; 32117973; 32122936; 32156754; 32157127; 32175841; 32184260; 32199908; 32219590; 32312516; 32328627; 32339165; 32376880; 32404863; 32445262; 32457905; 32464117; 32485126; 32521639; 32531091; 32540914; 32598968; 32641420; 32658993; 32661924; 32737445; 32738610; 32791328; 32888651; 32917927; 32993812; 33022230; 33028612; 33060329; 33063715; 33065339; 33100975; 33135631; 33203680; 33233821; 33288705; 33299007; 33309868; 33320356; 33358662; 33377870; 33404503; 33425686; 33464406; 33547995; 33558234; 33561221; 33593820; 33616626; 33662357; 33672471; 33800390; 33802079; 33824299; 33863891; 33920158; 33983927; 34197724; 34200604; 34313318; 34411095; 34453033; 34463269; 5004680; 5004809; 6414457; 6799259; 6799353; 6801435; 7720583; 7916308; 7982560; 8077114; 8325246; 8601490; 8660866; 8719770; 8805273; 8964464; 9017901; 9144214; 9196327; 9294184; 9335599; 9409683; 9409831; 9435287; 9463354; 9545255; 9584170; 9612077; 9636184; 9814699; 9814703; 9814704; 9852042; 9862464; 9874786; 9876186; 9916984; 9916987; 9988218; 9988219; 9990849;
Motif
Gene Encoded By
Mass 48,038
Kinetics
Metal Binding METAL 263; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00029; METAL 266; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00029; METAL 283; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00029; METAL 290; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00029
Rhea ID
Cross Reference Brenda