| IED ID | IndEnz0002014287 |
| Enzyme Type ID | protease014287 |
| Protein Name |
Protease Do-like 1, chloroplastic EC 3.4.21.- Protein DEGRADATION OF PERIPLASMIC PROTEINS 1 DEGP PROTEASE 1 |
| Gene Name | DEGP1 DEG1 DEGP At3g27925 K16N12.18 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MATTTSCSLLLSSTLFLHSPPSSHLSFFNLSSSRSSPISLYPIRSKRYFRILSKLSLNDNNRDDDDDTLHFTPFSAVKPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEVLRGDHKEKISVTLEPKPDES |
| Enzyme Length | 439 |
| Uniprot Accession Number | O22609 |
| Absorption | |
| Active Site | ACT_SITE 173; /note=Charge relay system; /evidence=ECO:0000255; ACT_SITE 203; /note=Charge relay system; /evidence=ECO:0000255; ACT_SITE 282; /note=Charge relay system; /evidence=ECO:0000255 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by phenylmethylsulfonyl fluoride and O-phenanthroline. {ECO:0000269|PubMed:9507020}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that is required at high temperature. May be involved in the degradation of damaged proteins. In vivo, can degrade beta-casein. {ECO:0000269|PubMed:21877139, ECO:0000269|PubMed:9507020}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (22); Chain (1); Domain (1); Helix (9); Mutagenesis (1); Region (1); Sequence conflict (9); Transit peptide (2); Turn (2) |
| Keywords | 3D-structure;Chloroplast;Direct protein sequencing;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Serine protease;Stress response;Thylakoid;Transit peptide |
| Interact With | Itself |
| Induction | INDUCTION: By heat shock. {ECO:0000269|PubMed:9507020}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:21877139, ECO:0000269|PubMed:9507020}; Peripheral membrane protein {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:9507020}; Lumenal side {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:9507020}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3QO6; |
| Mapped Pubmed ID | 11826309; 12376650; 14617066; 14670603; 15028209; 15266057; 15545296; 16648217; 16895613; 17181860; 17351117; 18431481; 20049866; 20088900; 21532594; 21798944; 22027029; 22520048; 23563498; 24064926; 25161662; 26909098; 27247031; 28627464; 29588501; 30237207; 31886945; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,674 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |