Detail Information for IndEnz0002014288
IED ID IndEnz0002014288
Enzyme Type ID protease014288
Protein Name Protease Do-like 2, chloroplastic
EC 3.4.21.-
Gene Name DEGP2 At2g47940 F17A22.33 T9J23.7
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAASVANCCFSVLNASVKIQSSSISSPWCFVSASSLTPRASSNIKRKSSRSDSPSPILNPEKNYPGRVRDESSNPPQKMAFKAFGSPKKEKKESLSDFSRDQQTDPAKIHDASFLNAVVKVYCTHTAPDYSLPWQKQRQFTSTGSAFMIGDGKLLTNAHCVEHDTQVKVKRRGDDRKYVAKVLVRGVDCDIALLSVESEDFWKGAEPLRLGHLPRLQDSVTVVGYPLGGDTISVTKGVVSRIEVTSYAHGSSDLLGIQIDAAINPGNSGGPAFNDQGECIGVAFQVYRSEETENIGYVIPTTVVSHFLTDYERNGKYTGYPCLGVLLQKLENPALRECLKVPTNEGVLVRRVEPTSDASKVLKEGDVIVSFDDLHVGCEGTVPFRSSERIAFRYLISQKFAGDIAEIGIIRAGEHKKVQVVLRPRVHLVPYHIDGGQPSYIIVAGLVFTPLSEPLIEEECEDTIGLKLLTKARYSVARFRGEQIVILSQVLANEVNIGYEDMNNQQVLKFNGIPIRNIHHLAHLIDMCKDKYLVFEFEDNYVAVLEREASNSASLCILKDYGIPSERSADLLEPYVDPIDDTQALDQGIGDSPVSNLEIGFDGLVWA
Enzyme Length 607
Uniprot Accession Number O82261
Absorption
Active Site ACT_SITE 159; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 190; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 268; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II. {ECO:0000269|PubMed:11179216}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (29); Chain (1); Compositional bias (2); Domain (1); Helix (13); Region (2); Transit peptide (2); Turn (4)
Keywords 3D-structure;Alternative splicing;Chloroplast;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Serine protease;Thylakoid;Transit peptide
Interact With
Induction INDUCTION: By high salt, desiccation and light stresses (at protein level). {ECO:0000269|PubMed:11179216}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:11179216}; Peripheral membrane protein {ECO:0000269|PubMed:11179216}; Stromal side {ECO:0000269|PubMed:11179216}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 4FLN; 5ILB;
Mapped Pubmed ID 11717304; 12185496; 12753585; 14576160; 14670603; 16895613; 17157840; 17181860; 17885809; 18431481; 18633119; 18650403; 18854047; 21139083; 21668884; 22008015; 22027029; 22520048; 22961982; 25161662; 26909098; 28627464; 29180814; 32663165;
Motif
Gene Encoded By
Mass 66,802
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda