| IED ID | IndEnz0002014294 |
| Enzyme Type ID | protease014294 |
| Protein Name |
Protease Do-like 8, chloroplastic EC 3.4.21.- |
| Gene Name | DEGP8 At5g39830 K13H13.1 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MQVIASFCSKPNENEFVGRRQLLSSVCSKISQGDVVSHPPVSSVKVTQDWKSNLHELAVKSVPSTTRRILLTSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKVLRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEEKSS |
| Enzyme Length | 448 |
| Uniprot Accession Number | Q9LU10 |
| Absorption | |
| Active Site | ACT_SITE 171; /note=Charge relay system; /evidence=ECO:0000255; ACT_SITE 214; /note=Charge relay system; /evidence=ECO:0000255; ACT_SITE 292; /note=Charge relay system; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Probable serine protease. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (18); Chain (1); Domain (1); Helix (8); Region (1); Transit peptide (2); Turn (3) |
| Keywords | 3D-structure;Alternative splicing;Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;Reference proteome;Serine protease;Thylakoid;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen {ECO:0000269|PubMed:11719511}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4IC6; |
| Mapped Pubmed ID | 11826309; 12185496; 14576160; 16648217; 16895613; 17181860; 17449806; 18431481; 18650403; 20089771; 22008015; 22027029; 22520048; 22698923; 22750879; 23295491; 23633592; 24862025; 25161662; 26909098; 28627464; 29367302; 30237207; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,493 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.107; |