| IED ID | IndEnz0002014328 |
| Enzyme Type ID | protease014328 |
| Protein Name |
Serine endoprotease DegS EC 3.4.21.107 Site-1 protease DegS S1P protease DegS Site-1-type intramembrane protease |
| Gene Name | degS STM14_4041 |
| Organism | Salmonella typhimurium (strain 14028s / SGSC 2262) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella typhimurium (strain 14028s / SGSC 2262) |
| Enzyme Sequence | MFVKLLRSVAIGLIVGAILLAVMPSLRKINPIAVPQFDSTDETPASYNFAVRRAAPAVVNVYNRSMNSTAHNQLEIRTLGSGVIMDQRGYIITNKHVINDADQIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINTKRTPHIGDVVLAIGNPYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTLSFDKSNDGETPEGLGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQQGSGMDPIQGIVVNEVTPNGPAALAGIQVNDLIISVNNKPAVSALETMDQVAEIRPGSVIPVVVMRDDKQLTFQVTVQEYPASN |
| Enzyme Length | 356 |
| Uniprot Accession Number | D0ZY51 |
| Absorption | |
| Active Site | ACT_SITE 96; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P0AEE4; ACT_SITE 126; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P0AEE4; ACT_SITE 201; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P0AEE4 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.; EC=3.4.21.107; |
| DNA Binding | |
| EC Number | 3.4.21.107 |
| Enzyme Function | FUNCTION: A site-1 protease (S1P) that cleaves the peptide bond between 'Val-148' and 'Ser-149' in RseA. When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porins (OMP) and then initiates RseA (anti sigma-E factor) degradation by cleaving its periplasmic domain, making it a substrate for subsequent cleavage by RseP. This cascade ultimately leads to the sigma-E-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly. Required for basal and heat shock-induced degradation of RseA but not for acid stress response in this strain. {ECO:0000269|PubMed:19170886}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Transmembrane (1) |
| Keywords | Cell inner membrane;Cell membrane;Hydrolase;Membrane;Protease;Serine protease;Transmembrane;Transmembrane helix;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,795 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |