| IED ID | IndEnz0002014360 |
| Enzyme Type ID | protease014360 |
| Protein Name |
Peptidyl-Asp metalloendopeptidase EC 3.4.24.33 Endopeptidase Asp-N |
| Gene Name | Smal_0815 |
| Organism | Stenotrophomonas maltophilia (strain R551-3) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Xanthomonadales Xanthomonadaceae Stenotrophomonas Stenotrophomonas maltophilia group Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia) Stenotrophomonas maltophilia (strain R551-3) |
| Enzyme Sequence | MLSRSIGKAAGGLVLGLSVAAAAHAAPLFEPVTVLSRAAAGSEPALGKLLATPSTATVQEVRVDAAATAQPQLEFELLGQRVQAVRSKVESLPDGGSIWYGQFRSPSDRLTAATSNGQDDPGNSVILVRSGNTVTGSIRKDGKLYRLRPLGNRHVLVEVDESRMPADHPADYNQLPKIPMGNNDRIGIAQASSGTPATIRVLVVATNAAVAAYGGNMQSLVQLAVAESNQGYVNSNVGITLQLAGYETTSYTESGNFTTDLTRFRNTSDGYMDSIHTSRNNTAADVGVLLINNTSYCGLASGIGSTASTAFAAVYWDCATGYYSFAHEIGHLQSARHDIASDPSTSPYAYGHGYRYEPASGSRWRTIMAYDCSAGCPRLNYWSNPNISYNGVPMGIASSADNQRVLVNTKATIAAFR |
| Enzyme Length | 417 |
| Uniprot Accession Number | B4SLL0 |
| Absorption | |
| Active Site | ACT_SITE 328; /evidence="ECO:0000250|UniProtKB:O75173, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of Xaa-|-Asp, Xaa-|-Glu and Xaa-|-cysteic acid bonds.; EC=3.4.24.33; Evidence={ECO:0000250|UniProtKB:Q9R4J4}; |
| DNA Binding | |
| EC Number | 3.4.24.33 |
| Enzyme Function | FUNCTION: Metalloprotease, specifically cleaves on the N-terminal side of aspartyl, glutamyl and cysteic acid residues. {ECO:0000250|UniProtKB:Q9R4J4}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Signal peptide (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Protease;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,067 |
| Kinetics | |
| Metal Binding | METAL 327; /note="Zinc; catalytic"; /evidence="ECO:0000250|UniProtKB:O75173, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 331; /note="Zinc; catalytic"; /evidence="ECO:0000250|UniProtKB:O75173, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 337; /note="Zinc; catalytic"; /evidence="ECO:0000250|UniProtKB:O75173, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Rhea ID | |
| Cross Reference Brenda |