| IED ID | IndEnz0002014371 |
| Enzyme Type ID | protease014371 |
| Protein Name |
Alkaline serine protease AkP EC 3.4.21.- Fragment |
| Gene Name | |
| Organism | Termitomyces clypeatus (White-rot fungus) (Sinotermitomyces taiwanensis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Lyophyllaceae Termitomyces Termitomyces clypeatus (White-rot fungus) (Sinotermitomyces taiwanensis) |
| Enzyme Sequence | GTQTNAPWGLARLIS |
| Enzyme Length | 15 |
| Uniprot Accession Number | C0HJJ8 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by serine protease inhibitors phenylmethanesulphonylfluoride (PMSF) and di-iodopropylfluorophosphate (DFP). {ECO:0000269|Ref.1}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Protease capable of hydrolyzing gelatin and cell surface heparan sulfate proteoglycans (HSPGs) in vitro. {ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9. Activity is relatively stable between pH 8-10 but drops at lower pH and is absent at pH 4. {ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Hydrolase;Protease;Secreted;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,585 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.7 mM for azocasein {ECO:0000269|Ref.1}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |