| IED ID | IndEnz0002014374 |
| Enzyme Type ID | protease014374 |
| Protein Name |
Astacin EC 3.4.24.21 Crayfish small molecule proteinase |
| Gene Name | |
| Organism | Astacus astacus (Noble crayfish) (Astacus fluviatilis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Crustacea Multicrustacea Malacostraca Eumalacostraca Eucarida Decapoda Pleocyemata Astacidea (true lobsters and crayfishes) Astacoidea (crayfish) Astacidae Astacus Astacus astacus (Noble crayfish) (Astacus fluviatilis) |
| Enzyme Sequence | MQCAVLLVLLGVVAASPIIPEAARALYYNDGMFEGDIKLRAGRQPARVGAAILGDEYLWSGGVIPYTFAGVSGADQSAILSGMQELEEKTCIRFVPRTTESDYVEIFTSGSGCWSYVGRISGAQQVSLQANGCVYHGTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNGIDLTDPYDKAHMLQTDANQINNLYTNECSLRH |
| Enzyme Length | 251 |
| Uniprot Accession Number | P07584 |
| Absorption | |
| Active Site | ACT_SITE 142; /evidence="ECO:0000255|PROSITE-ProRule:PRU01211, ECO:0000305|PubMed:1319561" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.; EC=3.4.24.21; Evidence={ECO:0000269|PubMed:2261483, ECO:0000269|Ref.3}; |
| DNA Binding | |
| EC Number | 3.4.24.21 |
| Enzyme Function | FUNCTION: Metalloprotease. This protease prefers to cleave in front of small aliphatic residues (P1'). The presence of Lys or Arg in the P1 and P2 position yields high-turnover substrates. In the P3 position the enzyme prefers Pro > Val > Leu > Ala > Gly. {ECO:0000269|PubMed:2261483}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (9); Chain (1); Disulfide bond (2); Domain (1); Helix (12); Metal binding (3); Propeptide (2); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..15; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (11) |
| Cross Reference PDB | 1AST; 1IAA; 1IAB; 1IAC; 1IAD; 1IAE; 1QJI; 1QJJ; 3LQ0; 6HT9; 6SAZ; |
| Mapped Pubmed ID | 20202938; 30867929; 31604990; 8006015; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,093 |
| Kinetics | |
| Metal Binding | METAL 141; /note="Zinc; via tele nitrogen; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01211, ECO:0000269|PubMed:1319561, ECO:0000269|PubMed:8756323"; METAL 145; /note="Zinc; via tele nitrogen; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01211, ECO:0000269|PubMed:1319561, ECO:0000269|PubMed:8756323"; METAL 151; /note="Zinc; via tele nitrogen; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01211, ECO:0000269|PubMed:1319561, ECO:0000269|PubMed:8756323" |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.21; |