| IED ID | IndEnz0002014412 |
| Enzyme Type ID | protease014412 |
| Protein Name |
Cysteine protease atg4da EC 3.4.22.- Autophagy-related protein 4 homolog D-A |
| Gene Name | atg4da si:ch211-212h16.5 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MNSVSPSAVQYIVGGGAHEDKASASSKRHLGHGAVPDGIREGSGEPDEVDRLKAKFMSAWNNVKYGWTVKSKTSFNKSSPLILLGQSFLFNNEDEVERFRQTFVSCVWLTYRREFPQLDGSSLTTDCGWGCMLRSGQMMLAQGLLLHLMPTDWRWSDCHALTDVDFEVLKPRSPSRPAGMSMPSFSSSWSSSIPQINPSPGITEAHRRAPARCPSASPDPQVDALHRKVVSCFGDHPSAPFGVHQLVELGKESGKRAGDWYGPSVVAHMLRKAVARAAEFEDLAVYVAQDCTVYKEDVMSLCESSGVGWKSVVILVPVRLGGESLNPSYIECVKNILKLKCCIGIIGGKPKHSLFFVGFQDEQLLYLDPHYCQPVVDVTQANFSLESFHCNSPRKMNFSRMDPSCTIGLYARSKTDFESLCTAVSEALSSSKEKYPIFTFVEGRGQIYGMEGPSGGSVDAPAHIFTCSRLSRNNKRGSTDEFVLL |
| Enzyme Length | 485 |
| Uniprot Accession Number | R4GER2 |
| Absorption | |
| Active Site | ACT_SITE 131; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9Y4P1; ACT_SITE 368; /evidence=ECO:0000250|UniProtKB:Q9Y4P1; ACT_SITE 370; /evidence=ECO:0000250|UniProtKB:Q9Y4P1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:67548, Rhea:RHEA-COMP:17323, Rhea:RHEA-COMP:17324, ChEBI:CHEBI:15377, ChEBI:CHEBI:64612, ChEBI:CHEBI:172940, ChEBI:CHEBI:172941; Evidence={ECO:0000250|UniProtKB:Q86TL0};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67549; Evidence={ECO:0000250|UniProtKB:Q86TL0}; CATALYTIC ACTIVITY: Reaction=[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine; Xref=Rhea:RHEA:67576, Rhea:RHEA-COMP:17324, Rhea:RHEA-COMP:17326, ChEBI:CHEBI:15377, ChEBI:CHEBI:57262, ChEBI:CHEBI:172940, ChEBI:CHEBI:172942; Evidence={ECO:0000250|UniProtKB:Q86TL0};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67577; Evidence={ECO:0000250|UniProtKB:Q86TL0}; |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (By similarity). The protease activity is required for proteolytic activation of ATG8 family proteins to reveal a C-terminal glycine (By similarity). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (By similarity). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (By similarity). ATG4D plays a role in the autophagy-mediated neuronal homeostasis in the central nervous system (PubMed:25875846). {ECO:0000250|UniProtKB:Q86TL0, ECO:0000250|UniProtKB:Q9Y4P1, ECO:0000269|PubMed:25875846}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Autophagy;Cytoplasm;Hydrolase;Protease;Protein transport;Reference proteome;Thiol protease;Transport |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86TL0}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 27171500; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,363 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:67548; RHEA:67549; RHEA:67576; RHEA:67577 |
| Cross Reference Brenda |