| IED ID | IndEnz0002014430 |
| Enzyme Type ID | protease014430 |
| Protein Name |
Cysteine protease atg4 EC 3.4.22.- Autophagy-related protein 4 |
| Gene Name | atg4-1 apg4-1 DDB_G0273443; atg4-2 apg4-2 DDB_G0273553 |
| Organism | Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
| Enzyme Sequence | MFTKYSHHNGYQDGSHLQPFQYQQQTYNQQSYLRQQQQAPQQISYGFNQPNSPTSSSSTPSSSTAMGNSFQNQRQINLQQQQQQEQFLQEQVFYQQQLLQQQSQIKEQQRQKEQKQKTNILNIYKEGKQKIMMSFYNLYRNYPTEPPHFSPSPIWLMGRCYTSKDNNSNNNSNNNQVPQTQPTQLQQSIGIFQNNNSNSNNNNNHNNNHNNNNNNLTTDLIYRPAIESGFLSDVASMIWFSYRKDFPPIENTNITTDIGWGCMLRTGQMILARALIKHLYKENDMVPEIERKKPHSNYSQVLAWFSDYPSKEHVYGIHQIVNKKQAMEKNNRKQQILREQVISLNRGGGGSSKGKKKKEKEEEINDNVEEWLAPTRISNILRQLIKFQHLEDLEMYVPTDGVIYKDYINNLCNNSNTHNHYQIIQQQLQHLREQQNIQQNNNKNNNNNNPTTTTTTTTTATSSNNNNNQSPPSRVPNGYNNQVFDDESLFDYNTAISSIPPKWKSLIIMIPLKLGADKLNSTYIEKLKLLLKLPQSLGFIGGKPKQSFYFIGFQDDQVIYLDPHFVQESVNPNSFDYSNTYSGCIPQKMPFTQLDPSLSIGFYCRDQASFEDLCDRLSVINNCEFPIISVCQKLPDYQIECELVDDYAESETTEMLAITIANGGNNHSCIPENIVVDDEEFIVHHHIPYNPNNNQNNNQNNNNNNNKNNNNNTNQQQTPNYPPKLNTYQPDFSSDGEIDDFTMVG |
| Enzyme Length | 745 |
| Uniprot Accession Number | Q557H7 |
| Absorption | |
| Active Site | ACT_SITE 262; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9Y4P1; ACT_SITE 562; /evidence=ECO:0000250|UniProtKB:Q9Y4P1; ACT_SITE 564; /evidence=ECO:0000250|UniProtKB:Q9Y4P1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:67548, Rhea:RHEA-COMP:17323, Rhea:RHEA-COMP:17324, ChEBI:CHEBI:15377, ChEBI:CHEBI:64612, ChEBI:CHEBI:172940, ChEBI:CHEBI:172941; Evidence={ECO:0000250|UniProtKB:Q9Y4P1};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67549; Evidence={ECO:0000250|UniProtKB:Q9Y4P1}; |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:12626495). The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine (By similarity). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of PE-conjugated ATG8 proteins (By similarity). {ECO:0000250|UniProtKB:Q9Y4P1, ECO:0000269|PubMed:12626495}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Region (5); Sequence conflict (1) |
| Keywords | Autophagy;Cytoplasm;Hydrolase;Protease;Protein transport;Reference proteome;Thiol protease;Transport;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14736886; 19079286; 20603609; 22722653; |
| Motif | |
| Gene Encoded By | |
| Mass | 86,136 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:67548; RHEA:67549 |
| Cross Reference Brenda |