| IED ID | IndEnz0002014477 |
| Enzyme Type ID | protease014477 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase BAP1 EC 3.4.19.12 BRCA1-associated protein 1 |
| Gene Name | BAP1 BRAFLDRAFT_277645 |
| Organism | Branchiostoma floridae (Florida lancelet) (Amphioxus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Cephalochordata Leptocardii Amphioxiformes Branchiostomidae Branchiostoma Branchiostoma floridae (Florida lancelet) (Amphioxus) |
| Enzyme Sequence | MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLSKPIEGPVYGFIFLFKWSEERRSRRKVGPSMEESFVVDEDIVNDMFFAQQLIPNSCATHALLSVLLNCPQISLGSTLTRLKYFTRGMGPESKGWAIGNVPEIARAHNSHARPEPRHLPEKQTGISSVRTAEAFHFVSYVTTNDRLFELDGLKPYPIDHGPWGEKEDWTEKFRRVISERIGNATGGENGHDIRFNLMAVVADRRQQYETKLNTLKTNRQIVLEALQQVGGVNTKKSIQYAEVSHVCLHPPKFVPCSDDCAIWDALRYSSALRCSLIQHGCEHPSIPRSDGSTLVSWPSCVTSVNIVVSGHLKYVLLCFSIILDHKLEKLVPARLDPIKVNEPHLTSLIPSAFGRESHHKSKASSTVTSSSAAASSSAAGTDGVQPAEMKPDEFLHPDASRRVGFLEMPWRSDEARPLSIHTGYSKIPSPAPSNESTDTASEIGSAFNSPVRSANRSALQTIPEGSITKQEGDTKTDANIITVCLKRSLSGEGGGPPAKRLHIGDPDMALQRLGSGDSVEGDPMALQGGSGTGDAQQDQHALQAGGARPNMAHAFAPKDLLALLKNVETEIQICEQHLKDEIEKRKKYKVDDCRRTHNYDDFIRTFLRMLAEQGQLSKLVDQHVLVRRRQGVSVGRLHKQRKPDRRRKRARVSDKYRRKKQ |
| Enzyme Length | 694 |
| Uniprot Accession Number | C4A0D9 |
| Absorption | |
| Active Site | ACT_SITE 91; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q92560; ACT_SITE 169; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P09936 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q92560}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (By similarity). {ECO:0000250|UniProtKB:Q92560}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (2); Motif (1); Region (3); Site (1) |
| Keywords | Chromatin regulator;Cytoplasm;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q92560}. Nucleus {ECO:0000250|UniProtKB:Q92560}. Note=Mainly nuclear. Binds to chromatin. {ECO:0000250|UniProtKB:Q92560}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 678..683; /note=Nuclear localization signal; /evidence=ECO:0000250|UniProtKB:Q92560 |
| Gene Encoded By | |
| Mass | 77,164 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |