| IED ID | IndEnz0002014574 |
| Enzyme Type ID | protease014574 |
| Protein Name |
Aspartic protease 2 Na-APR-2 EC 3.4.23.- Necepsin I Pepsin-like aspartic protease 2 |
| Gene Name | apr-2 ncpI |
| Organism | Necator americanus (Human hookworm) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Strongylida Ancylostomatoidea Ancylostomatidae Bunostominae Necator Necator americanus (Human hookworm) |
| Enzyme Sequence | MRSILVLVALIGCIAAGVYKIPLKRITPPMIKMLRAGTWETYVEGMRKRQLQLLKEHKVHIQDVLGYANMEYLGEITIGTPQQKFLVVLDTGSSNLWVPDDSCYKEKRPDRCLVSNCDAGLVCQVFCPDPKCCEHTREFKQVNACKDKHRFDQKNSNTYVKTNKTWAIAYGTGDARGFFGRDTVRLGAEGKDQLVINDTWFGQAEHIAEFFSNTFLDGILGLAFQELSEGGVAPPIIRAIDLGLLDQPIFTVYFENVGDKEGVYGGVFTWGGLDPDHCEDEVTYEQLTEATYWQFRLKGVSSKNFSSTAGWEAISDTGTSLNGAPRGILRSIARQYNGQYVASQGLYVVDCSKNVTVDVTIGDRNYTMTAKNLVLEIQADICIMAFFEMDMFIGPAWILGDPFIREYCNIHDIEKKRIGFAAVKH |
| Enzyme Length | 425 |
| Uniprot Accession Number | Q9N9H4 |
| Absorption | |
| Active Site | ACT_SITE 90; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 316; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by pepstatin A. {ECO:0000269|PubMed:12552433}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Aspartic protease which cleaves several human serum proteins including hemoglobin, fibrinogen and albumin. Appears to cleave preferentially between P1 (Ala, Leu, Val, Phe and Gly) and P1' (Ala and Leu) residues. {ECO:0000269|PubMed:12552433}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5. {ECO:0000269|PubMed:12552433}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Aspartyl protease;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12552433}. Note=Secreted into the gut lumen. {ECO:0000269|PubMed:12552433}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved into a mature form. {ECO:0000269|PubMed:12552433}. |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,650 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |