| IED ID | IndEnz0002014593 |
| Enzyme Type ID | protease014593 |
| Protein Name |
Beta carbonic anhydrase 1, chloroplastic AtbCA1 AtbetaCA1 EC 4.2.1.1 Beta carbonate dehydratase 1 Protein SALICYLIC ACID-BINDING PROTEIN 3 AtSABP3 |
| Gene Name | BCA1 CA1 SABP3 At3g01500 F4P13.5 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSTAPLSGFFLTSLSPSQSSLQKLSLRTSSTVACLPPASSSSSSSSSSSSRSVPTLIRNEPVFAAPAPIIAPYWSEEMGTEAYDEAIEALKKLLIEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFIKFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPPFDKVKYGGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKICLPAKSKVISELGDSAFEDQCGRCEREAVNVSLANLLTYPFVREGLVKGTLALKGGYYDFVKGAFELWGLEFGLSETSSVKDVATILHWKL |
| Enzyme Length | 347 |
| Uniprot Accession Number | P27140 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H(+) + hydrogencarbonate = CO2 + H2O; Xref=Rhea:RHEA:10748, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17544; EC=4.2.1.1; Evidence={ECO:0000255|RuleBase:RU003956}; |
| DNA Binding | |
| EC Number | 4.2.1.1 |
| Enzyme Function | FUNCTION: Reversible hydration of carbon dioxide. Required for photosynthesis in cotyledons. Binds salicylic acid. Together with BCA4, involved in the CO(2) signaling pathway which controls gas-exchange between plants and the atmosphere by modulating stomatal development and movements. Promotes water use efficiency. {ECO:0000269|PubMed:18434607, ECO:0000269|PubMed:19017644, ECO:0000269|PubMed:20010812, ECO:0000269|PubMed:25043023}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Chain (1); Erroneous initiation (1); Modified residue (5); Mutagenesis (1); Transit peptide (1) |
| Keywords | Acetylation;Alternative splicing;Cell membrane;Chloroplast;Lyase;Membrane;Phosphoprotein;Plastid;Reference proteome;S-nitrosylation;Transit peptide;Zinc |
| Interact With | |
| Induction | INDUCTION: Expression reduced by 70% under dark conditions. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:17407539}. Cell membrane {ECO:0000269|PubMed:20010812}; Peripheral membrane protein {ECO:0000269|PubMed:20010812}. |
| Modified Residue | MOD_RES 114; /note=N-acetylalanine; /evidence=ECO:0007744|PubMed:22223895; MOD_RES 175; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P42737; MOD_RES 203; /note=Phosphotyrosine; /evidence=ECO:0007744|PubMed:22092075; MOD_RES 266; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22092075; MOD_RES 280; /note=S-nitrosocysteine; /evidence=ECO:0000269|PubMed:19017644 |
| Post Translational Modification | PTM: S-nitrosylation at Cys-280 is up-regulated during nitrosative burst and suppresses both binding of salicylic acid and carbonic anhydrase activity. S-nitrosylated in response to an avirulent but not to a virulent bacterial strain. {ECO:0000269|PubMed:19017644}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11826309; 12766230; 12938931; 14535880; 15028209; 15322131; 15352244; 16207701; 16648217; 16923014; 17028151; 17432890; 17899171; 18431481; 18538804; 18633119; 18650403; 18818313; 19448040; 19857612; 21798944; 22442409; 22995300; 23281391; 24211190; 24913047; 26243620; 26482706; 26829901; 26889912; 27122571; 27170143; 27473496; 28522549; 28627464; 28753666; 28988531; 29090655; 31308150; 31889067; 32146566; 32344669; 32751472; 8029363; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,450 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10748 |
| Cross Reference Brenda |