| IED ID | IndEnz0002014615 |
| Enzyme Type ID | protease014615 |
| Protein Name |
Cysteine proteinase EP-B 2 EC 3.4.22.- |
| Gene Name | EPB2 |
| Organism | Hordeum vulgare (Barley) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
| Enzyme Sequence | MGLLSKKLLVASMVAAVLAVAAVELCSAIPMEDKDLESEEALWDLYERWQSAHRVRRHHAEKHRRFGTFKSNAHFIHSHNKRGDHPYRLHLNRFGDMDQAEFRATFVGDLRRDTPSKPPSVPGFMYAALNVSDLPPSVDWRQKGAVTGVKDQGKCGSCWAFSTVVSVEGINAIRTGSLVSLSEQELIDCDTADNDGCQGGLMDNAFEYIKNNGGLITEAAYPYRAARGTCNVARAAQNSPVVVHIDGHQDVPANSEEDLARAVANQPVSVAVEASGKAFMFYSEGVFTGECGTELDHGVAVVGYGVAEDGKAYWTVKNSWGPSWGEQGYIRVEKDSGASGGLCGIAMEASYPVKTYSKPKPTPRRALGARESL |
| Enzyme Length | 373 |
| Uniprot Accession Number | P25250 |
| Absorption | |
| Active Site | ACT_SITE 158; /evidence=ECO:0000250; ACT_SITE 297; /evidence=ECO:0000250; ACT_SITE 318; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (8); Chain (1); Disulfide bond (3); Glycosylation (1); Helix (8); Propeptide (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Disulfide bond;Germination;Glycoprotein;Hydrolase;Protease;Signal;Thiol protease;Zymogen |
| Interact With | |
| Induction | INDUCTION: Synthesized by the aleurone cells stimulated by gibberellic acid. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2FO5; |
| Mapped Pubmed ID | 16793521; |
| Motif | |
| Gene Encoded By | |
| Mass | 40,511 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |