| IED ID | IndEnz0002014639 |
| Enzyme Type ID | protease014639 |
| Protein Name |
ATP-dependent zinc metalloprotease FTSH 2, chloroplastic AtFTSH2 EC 3.4.24.- Protein VARIEGATED 2 |
| Gene Name | FTSH2 VAR2 At2g30950 F7F1.16 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAASSACLVGNGLSVNTTTKQRLSKHFSGRQTSFSSVIRTSKVNVVKASLDGKKKQEGRRDFLKILLGNAGVGLVASGKANADEQGVSSSRMSYSRFLEYLDKDRVNKVDLYENGTIAIVEAVSPELGNRVERVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSVLFNLIGNLAFPALLIGGLFLLSRRSGGGMGGPGGPGNPLQFGQSKAKFQMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGAKIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVPDVKGRTDILKVHAGNKKFDNDVSLEIIAMRTPGFSGADLANLLNEAAILAGRRARTSISSKEIDDSIDRIVAGMEGTVMTDGKSKSLVAYHEVGHAVCGTLTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEIIFGDSEVTTGAVGDLQQITGLARQMVTTFGMSDIGPWSLMDSSAQSDVIMRMMARNSMSEKLAEDIDSAVKKLSDSAYEIALSHIKNNREAMDKLVEVLLEKETIGGDEFRAILSEFTEIPPENRVPSSTTTTPASAPTPAAV |
| Enzyme Length | 695 |
| Uniprot Accession Number | O80860 |
| Absorption | |
| Active Site | ACT_SITE 489; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions, but not involved in thermotolerance. {ECO:0000269|PubMed:10849347, ECO:0000269|PubMed:11717304, ECO:0000269|PubMed:14630971, ECO:0000269|PubMed:16972866}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 267..274; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (1); Chain (1); Compositional bias (1); Erroneous initiation (1); Metal binding (3); Nucleotide binding (1); Region (1); Topological domain (2); Transit peptide (2); Transmembrane (1) |
| Keywords | ATP-binding;Chloroplast;Developmental protein;Direct protein sequencing;Hydrolase;Membrane;Metal-binding;Metalloprotease;Nucleotide-binding;Plastid;Protease;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix;Ubl conjugation;Zinc |
| Interact With | |
| Induction | INDUCTION: By cold and high light. {ECO:0000269|PubMed:15266057}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:14630971, ECO:0000269|PubMed:18431481}; Single-pass membrane protein {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:14630971}; Stromal side {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:14630971}. |
| Modified Residue | |
| Post Translational Modification | PTM: The FTSH2 precursor is ubiquitinated by CHIP in the cytoplasm. {ECO:0000269|PubMed:17714429}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11134419; 12167156; 12938931; 14729914; 15028209; 15304652; 15322131; 15803409; 16040665; 16157880; 16449381; 16603408; 16648217; 17263779; 17416734; 17432890; 17449646; 17543883; 18296449; 18599582; 18633119; 18775970; 19228339; 19669593; 19755395; 19767385; 19897671; 20023407; 20400527; 20666930; 20935174; 21062893; 21139083; 21187014; 21214651; 21438879; 21735825; 21798944; 22010036; 22121866; 22558304; 22579628; 22698923; 23299325; 23548781; 23703455; 23721655; 23802992; 24064926; 24124904; 24862025; 25893898; 25999408; 26435530; 26734046; 26909098; 27208291; 27303039; 27377564; 27535792; 28706530; 28808107; 29142022; 29973948; 30131421; 30915096; 30989223; 31160506; 31552075; 31836664; 31886945; 32210982; 32663165; 34072887; 8910594; 9987930; |
| Motif | |
| Gene Encoded By | |
| Mass | 74,157 |
| Kinetics | |
| Metal Binding | METAL 488; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 492; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 566; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B20; |