| IED ID | IndEnz0002014685 |
| Enzyme Type ID | protease014685 |
| Protein Name |
ATP-dependent protease subunit HslV EC 3.4.25.2 |
| Gene Name | hslV HDEF_1271 |
| Organism | Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Enterobacteriaceae incertae sedis ant tsetse mealybug aphid etc. endosymbionts aphid secondary symbionts Candidatus Hamiltonella Candidatus Hamiltonella defensa Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Enzyme Sequence | MTTIVSVRRHNHVVIGGDGQVTLGNTVMKGNAKKVRRLHHDKVIAGFAGGTADAFTLFELFEAKLKLCSGQLTKAAVEMAKDWRTDRMLRRLEALLAVADETASLIITGNGDVIQPEDDLIAIGSGGPYAQASARALLENTNLSAREIVEKSLTIAGNICIYTNQYPTIEELKIKTKG |
| Enzyme Length | 178 |
| Uniprot Accession Number | C4K5T1 |
| Absorption | |
| Active Site | ACT_SITE 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00248 |
| Activity Regulation | ACTIVITY REGULATION: Allosterically activated by HslU binding. {ECO:0000255|HAMAP-Rule:MF_00248}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP-dependent cleavage of peptide bonds with broad specificity.; EC=3.4.25.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00248}; |
| DNA Binding | |
| EC Number | 3.4.25.2 |
| Enzyme Function | FUNCTION: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. {ECO:0000255|HAMAP-Rule:MF_00248}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3) |
| Keywords | Allosteric enzyme;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome;Sodium;Threonine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00248}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 19,234 |
| Kinetics | |
| Metal Binding | METAL 157; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248; METAL 160; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248; METAL 163; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248 |
| Rhea ID | |
| Cross Reference Brenda |