Detail Information for IndEnz0002014858
IED ID IndEnz0002014858
Enzyme Type ID protease014858
Protein Name ATP-dependent protease subunit HslV
EC 3.4.25.2
Gene Name hslV PFL_0437
Organism Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas fluorescens group (fluorescent pseudomonads) Pseudomonas protegens Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Enzyme Sequence MTTIVSVRRHGKVVMGGDGQVSLGNTVMKGNAKKVRRLYHGQVIAGFAGATADAFTLFERFEGQLEKHQGHLVRAAVELAKEWRTDRSLSRLEAMLAVANKDASLIITGNGDVVEPEDGLIAMGSGGAYAQAAASALLKKTDLSAREIVETALGIAGDICVFTNHTQTIEEQDLAE
Enzyme Length 176
Uniprot Accession Number Q4KJK2
Absorption
Active Site ACT_SITE 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00248
Activity Regulation ACTIVITY REGULATION: Allosterically activated by HslU binding. {ECO:0000255|HAMAP-Rule:MF_00248}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=ATP-dependent cleavage of peptide bonds with broad specificity.; EC=3.4.25.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00248};
DNA Binding
EC Number 3.4.25.2
Enzyme Function FUNCTION: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. {ECO:0000255|HAMAP-Rule:MF_00248}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Metal binding (3)
Keywords Allosteric enzyme;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome;Sodium;Stress response;Threonine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00248}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 18,721
Kinetics
Metal Binding METAL 157; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248; METAL 160; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248; METAL 163; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248
Rhea ID
Cross Reference Brenda