Detail Information for IndEnz0002014864
IED ID IndEnz0002014864
Enzyme Type ID protease014864
Protein Name ATP-dependent protease subunit HslV
EC 3.4.25.2
Gene Name hslV Pmen_0533
Organism Pseudomonas mendocina (strain ymp)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas mendocina Pseudomonas mendocina (strain ymp)
Enzyme Sequence MTTIVSVRRHGKVVMAGDGQVSLGNTVMKGNAKKVRRLYHGQVLAGFAGATADAFTLFERFEGQLEKHQGHLVRAAVELAKDWRTDRSLSRLEAMLAVANKDASLIITGNGDVVEPEEGLIAMGSGGNFAQAAARALLMKTDLSALEVAQTALGIAGDICVFTNHHQTIEELDAAE
Enzyme Length 176
Uniprot Accession Number A4XPN7
Absorption
Active Site ACT_SITE 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00248
Activity Regulation ACTIVITY REGULATION: Allosterically activated by HslU binding. {ECO:0000255|HAMAP-Rule:MF_00248}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=ATP-dependent cleavage of peptide bonds with broad specificity.; EC=3.4.25.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00248};
DNA Binding
EC Number 3.4.25.2
Enzyme Function FUNCTION: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. {ECO:0000255|HAMAP-Rule:MF_00248}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Metal binding (3)
Keywords Allosteric enzyme;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome;Sodium;Threonine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00248}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 18,727
Kinetics
Metal Binding METAL 157; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248; METAL 160; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248; METAL 163; /note=Sodium; via carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00248
Rhea ID
Cross Reference Brenda