| IED ID |
IndEnz0002015271 |
| Enzyme Type ID |
protease015271 |
| Protein Name |
Elastase inhibitor AFLEI
|
| Gene Name |
|
| Organism |
Aspergillus flavus |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Pezizomycotina
leotiomyceta
Eurotiomycetes
Eurotiomycetidae
Eurotiales (green and blue molds)
Aspergillaceae
Aspergillus
Aspergillus subgen. Circumdati
Aspergillus flavus
|
| Enzyme Sequence |
DPATCEKEAQFVKQELIGQPYTDAVANALQSNPIRVLHPGDMITMEYIASRLNIQVNENNEIISAHCA |
| Enzyme Length |
68 |
| Uniprot Accession Number |
P86036 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
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| Enzyme Function |
FUNCTION: Elastase inhibitor. Inhibitor of A.flavus elastase with a Ki of 40 nM. Inhibitor of A.fumigatus elastase and human leukocyte elastase. Inhibits the fibrinogenase and collagenase activities of A.flavus elastase. Does not inhibit porcine pancreatic elastase, trypsin, chymotrypsin, thrombin or A.acutus AC1-proteinase. {ECO:0000269|PubMed:16940957, ECO:0000269|PubMed:17287718}. |
| Temperature Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Stable at temperatures below 80 degrees Celsius. {ECO:0000269|PubMed:16940957}; |
| PH Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Stable from pH 6 to 10. {ECO:0000269|PubMed:16940957}; |
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Disulfide bond (1) |
| Keywords |
Direct protein sequencing;Disulfide bond;Protease inhibitor;Secreted;Serine protease inhibitor |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
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| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
7,525 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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