| IED ID |
IndEnz0002015272 |
| Enzyme Type ID |
protease015272 |
| Protein Name |
Elastase inhibitor AFUEI
|
| Gene Name |
AFUA_3G14940 |
| Organism |
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Pezizomycotina
leotiomyceta
Eurotiomycetes
Eurotiomycetidae
Eurotiales (green and blue molds)
Aspergillaceae
Aspergillus
Aspergillus subgen. Fumigati
Neosartorya fumigata (Aspergillus fumigatus)
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
|
| Enzyme Sequence |
MKFSLACLLALAGLQAALADPATCEKEAQFVKQELIGQPYTDAVANALQSNPIRVLHPGDMITMEYIASRLNIQVNENNEIISAHCA |
| Enzyme Length |
87 |
| Uniprot Accession Number |
Q4WZ11 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Elastase inhibitor. Non-competitive inhibitor of A.fumigatus elastase with a Ki of 1.6 nM. Inhibits the fibrinogenase and collagenase activities of A.fumigatus elastase. Inhibitor of A.flavus elastase and human leukocyte elastase, and weakly inhibits porcine pancreatic elastase. Does not inhibit elastase from P.aeruginosa or C.atrox venom. {ECO:0000269|PubMed:18566136}. |
| Temperature Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Stable to heat treatment. Retains 33% of activity when heated to 100 degrees Celsius for 10 minutes at pH 7.5. {ECO:0000269|PubMed:18566136}; |
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (4); Chain (1); Disulfide bond (1); Helix (2); Signal peptide (1); Turn (1) |
| Keywords |
3D-structure;Direct protein sequencing;Disulfide bond;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18566136}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..19; /evidence=ECO:0000269|PubMed:18566136 |
| Structure 3D |
X-ray crystallography (2) |
| Cross Reference PDB |
3W0D;
3W0E;
|
| Mapped Pubmed ID |
23640894;
|
| Motif |
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| Gene Encoded By |
|
| Mass |
9,413 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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