| IED ID | IndEnz0002015295 |
| Enzyme Type ID | protease015295 |
| Protein Name |
Calpastatin Calpain inhibitor Cleaved into: Erythrocyte calpastatin |
| Gene Name | CAST |
| Organism | Oryctolagus cuniculus (Rabbit) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Lagomorpha Leporidae (rabbits and hares) Oryctolagus Oryctolagus cuniculus (Rabbit) |
| Enzyme Sequence | MNPAEAKAVPISKEMEGPHPHSKKRHRRQDAKTEPEKSQSTKPPVDHEKKAQEGKPKEHTKPKSTHKHASDGEGKHGRNEKTASRSKEPVTPAKRTEPETKPQDTKPAGGKSVAAGTTAAPGKAGDPKKEKKSLPAAALAEPKPDEPSGKSGMDAALDDLIDTLGEPSETQEDSTAYTGPEISDPMSSTYIEELGKREVTIPPKYRELLEKKTGVAGPPPDSVTPLGPDDAIDALSSDFTCSSPVASGKEAGKEAAKSAGEVLEAESAKVMRAAAPPQEKKRKVEEDAMSDQALEALSASLGTRMAEPELDLSSIKEVAEAKRKEEKVEKCGEDDETVPAEYRLKPATDKDGKPLLPEPAEKPKPRSESELIDELSKDFSQAKSNEKQPKPTGKTEESKAAVPAPVAEAVPRTSMCSIQPVPPKPASLQKSTVPDDAVEALAGSLGRKEADPEEGKPVADKIKEKSKEEEREKLGEKEETIPPDYRLEEAKDKDGKPLLPSEPTAQLPALSEDLLLDALSEDFSGPSSASSLKFDDAMLSAAVSEVVSQSPASITRATAPPPDTRPSNKELDDALDKLSDSLGQRQPDPDENKPMEDKVKERAKKEHKDKLGERDDTIPPEYRHLLDQGEQDKPEKPPTKKSKEIKKPAGDQDPIDALSGDLDSCPPAAETSQATEKDKSKTTTASSSKAAKHGDKAKDSAQTTEETSKPKANEKNAS |
| Enzyme Length | 718 |
| Uniprot Accession Number | P08855 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (2); Compositional bias (9); Cross-link (1); Modified residue (14); Region (5); Repeat (4) |
| Keywords | Acetylation;Direct protein sequencing;Isopeptide bond;Phosphoprotein;Protease inhibitor;Reference proteome;Repeat;Thiol protease inhibitor;Ubl conjugation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 49; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P51125; MOD_RES 86; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P51125; MOD_RES 133; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 222; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P51125; MOD_RES 243; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 290; /note=Blocked amino end (Ser); in form erythrocyte; MOD_RES 367; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 369; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 376; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 444; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 520; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 531; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 579; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810; MOD_RES 581; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P20810 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 76,965 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |