| IED ID |
IndEnz0002015319 |
| Enzyme Type ID |
protease015319 |
| Protein Name |
Subtilisin-chymotrypsin inhibitor WSCI
|
| Gene Name |
|
| Organism |
Triticum aestivum (Wheat) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Viridiplantae
Streptophyta
Streptophytina
Embryophyta
Tracheophyta
Euphyllophyta
Spermatophyta
Magnoliopsida
Mesangiospermae
Liliopsida
Petrosaviidae
commelinids
Poales
Poaceae
BOP clade
Pooideae
Triticodae
Triticeae
Triticinae
Triticum
Triticum aestivum (Wheat)
|
| Enzyme Sequence |
MSSVVKKPLGGNTDTGDHHNQKTEWPELVGKSVEEAKKVILQDKSEAQIVVLPVGTIVTMEYRIDRVRLFVDSLDKIAQVPRVG |
| Enzyme Length |
84 |
| Uniprot Accession Number |
P82977 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
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| Enzyme Function |
FUNCTION: Inhibits B.lichenoformis subtilisin, B.subtilis subtilisin, bovine pancreatic alpha-chymotrypsin and porcine alpha-chymotrypsin with Ki of 3.92 nM, 5.70 nM, 7.24 nM and 9.35 nM respectively. B.lichenoformis subtilisin is inhibited with a molar ratio of 1:0.87. Also inhibits chymotrypsin-like activities from the digestive tracts of the insect larvae T.molitor, P.interpunctella and H.armigera. Does not inhibit bovine pancreatic trypsin, porcine pancreatic elastase, or human leukocyte elastase. {ECO:0000269|PubMed:12675523, ECO:0000269|PubMed:15899701}. |
| Temperature Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 25 degrees Celsius when incubation is carried out at pH 7.0-9.0. {ECO:0000269|PubMed:12675523}; |
| PH Dependency |
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| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Region (1); Sequence conflict (2); Signal peptide (1); Site (1) |
| Keywords |
Direct protein sequencing;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal |
| Interact With |
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| Induction |
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| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue |
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| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..12; /evidence=ECO:0000269|PubMed:12675523 |
| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
9,326 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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