| IED ID | IndEnz0002015328 |
| Enzyme Type ID | protease015328 |
| Protein Name |
Indian hedgehog protein IHH Cleaved into: Indian hedgehog protein N-product; Indian hedgehog protein C-product |
| Gene Name | IHH |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MKPARLLLLLSGCALLLAPAVRCCGPGRVVGSRRRPPRKLIPLAYKQFSPNVPEKTLGASGRYEGKIARNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGMLARLAVEAGFDWVYYESKAHIHCSVKSEHSAAAKTGGCFPGRALATLENGARTPLWALRPGQRVLAMDGAGRPTYSDFLAFLDKEPRALTAFHVIETRQPPRRLALTPTHLLFVADNASAPAAQFRPTFASHVQPGHFVLVAVGSGGLQPAEVVGVRGRTDVGAYAPLTRHGTLVVDDVVASCFALVREQQLAQMAFWPLRLYHSLLGGPGVQGDGVHWYSGLLYRLGRMLLPPDSFHPLGAPRAES |
| Enzyme Length | 408 |
| Uniprot Accession Number | Q98938 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Lipidation (2); Metal binding (12); Signal peptide (1); Site (4) |
| Keywords | Autocatalytic cleavage;Calcium;Cell membrane;Developmental protein;Hydrolase;Lipoprotein;Membrane;Metal-binding;Palmitate;Protease;Reference proteome;Secreted;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Indian hedgehog protein N-product]: Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Extracellular side {ECO:0000250}. Note=The N-terminal peptide remains associated with the cell surface. {ECO:0000250}.; SUBCELLULAR LOCATION: [Indian hedgehog protein C-product]: Secreted, extracellular space {ECO:0000250}. Note=The C-terminal peptide diffuses from the cell. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity). {ECO:0000250}.; PTM: Cholesterylation is required for N-product targeting to lipid rafts and multimerization. {ECO:0000250}.; PTM: N-palmitoylation is required for N-product multimerization and full activity. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12968668; 14991716; 17889828; 21683695; 23928032; |
| Motif | |
| Gene Encoded By | |
| Mass | 44,829 |
| Kinetics | |
| Metal Binding | METAL 90; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 91; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 91; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 96; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 126; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 127; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 127; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 130; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 132; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 141; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 148; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 183; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q14623 |
| Rhea ID | |
| Cross Reference Brenda |