| IED ID | IndEnz0002015332 |
| Enzyme Type ID | protease015332 |
| Protein Name |
Indian hedgehog B protein IHHB Echidna hedgehog protein EHH Cleaved into: Indian hedgehog B protein N-product; Indian hedgehog B protein C-product |
| Gene Name | ihhb ehh ihh |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MRLSTAAALLTGFILAFSPAYDGCGPGRGYGKRRTPRKLTPLAYKQFSPNVAEKTLGASGRYEGKVTPSSERFKELTPNYNPDIIFKDEENTGADRMMTQRCKDKLNSLAISVMNLWPGVRLRVTEGWDEDGLHSEESLHYEGRAVDITTSDRDRNKYRMLARLAVEAGFDWVYYESKGHVHCSVKSEHSVAAKTGGCFPGRALVTMKDGSHRQIRDLQAGDLVLASEGSDGTGDLIYSEVLTFLDRRPITQKHFYVIRTEDGASVSLTAAHLLFMRVGNCSNRGEPKPGAVRTIFASDAQVGQCLLLGKLRKRFSQITHVGVREDQGLYPPLTAHGTVVVNDVLTSCYAAVNRQRLAHWAFAPLRLLYSWTGPDQVLKNGLHWYSQVLIGLGKLLLDSELFHPLALEATER |
| Enzyme Length | 412 |
| Uniprot Accession Number | Q98862 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning and muscle pioneer differentiation. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Lipidation (2); Metal binding (12); Sequence conflict (1); Signal peptide (1); Site (4) |
| Keywords | Autocatalytic cleavage;Calcium;Cell membrane;Developmental protein;Hydrolase;Lipoprotein;Membrane;Metal-binding;Palmitate;Protease;Reference proteome;Secreted;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Indian hedgehog B protein N-product]: Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Extracellular side {ECO:0000250}. Note=The N-terminal peptide remains associated with the cell surface. {ECO:0000250}.; SUBCELLULAR LOCATION: [Indian hedgehog B protein C-product]: Secreted, extracellular space {ECO:0000250}. Note=The C-terminal peptide diffuses from the cell. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity). {ECO:0000250}.; PTM: Cholesterylation is required for N-product targeting to lipid rafts and multimerization. {ECO:0000250}.; PTM: N-palmitoylation is required for N-product multimerization and full activity. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11428131; 23345245; |
| Motif | |
| Gene Encoded By | |
| Mass | 45,746 |
| Kinetics | |
| Metal Binding | METAL 89; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 90; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 90; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 95; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 125; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 126; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 126; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 129; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 131; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 140; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 147; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q14623; METAL 182; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q14623 |
| Rhea ID | |
| Cross Reference Brenda |