| IED ID | IndEnz0002015451 |
| Enzyme Type ID | protease015451 |
| Protein Name |
Signal peptidase I W SPase I EC 3.4.21.89 Leader peptidase I |
| Gene Name | sipW yqhE BSU24630 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MKLISNILYVIIFTLIIVLTLVVISTRSSGGEPAVFGYTLKSVLSGSMEPEFNTGSLILVKEITDVKELQKGDVITFMQDANTAVTHRIVDITKQGDHLLFKTKGDNNAAADSAPVSDENVRAQYTGFQLPYAGYMLHFASQPIGTAVLLIVPGVMLLVYAFVTISSAIREIERKTKALETDTKDSTMST |
| Enzyme Length | 190 |
| Uniprot Accession Number | P54506 |
| Absorption | |
| Active Site | ACT_SITE 45; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.; EC=3.4.21.89; Evidence={ECO:0000269|PubMed:9694797}; |
| DNA Binding | |
| EC Number | 3.4.21.89 |
| Enzyme Function | FUNCTION: Required for the cleavage of the signal sequence of TasA and TapA, which are involved in biofilm formation. {ECO:0000269|PubMed:10049401, ECO:0000269|PubMed:10559173, ECO:0000269|PubMed:16430695}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Transmembrane (2) |
| Keywords | Cell membrane;Hydrolase;Membrane;Protease;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Part of the tapA-sipW-tasA operon (PubMed:10464223). Expression is directly repressed by the DNA-binding protein master regulator of biofilm formation SinR and activated by the extracellular matrix regulatory protein RemA (PubMed:16430695, PubMed:23646920). Expressed constitutively at a low level (PubMed:9694797). Also positively regulated by the sporulation transcription factors sigma H and Spo0A and repressed by the transition phase regulatory protein AbrB, probably indirectly (PubMed:10464223). {ECO:0000269|PubMed:10464223, ECO:0000269|PubMed:16430695, ECO:0000269|PubMed:23646920, ECO:0000269|PubMed:9694797}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 20,678 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |