| IED ID | IndEnz0002015461 |
| Enzyme Type ID | protease015461 |
| Protein Name |
Aspartic proteinase NANA, chloroplast EC 3.4.23.- |
| Gene Name | NANA At3g12700 MBK21.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MYINTLFWKQNPTGDKKNQEEKMQKTLLSCLITTLLLITVADSMKDTSVRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTVGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCRYRARGKDNRRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIFGSSRSTKTAFRRTTPLDLTRIPPFYAINVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSACT |
| Enzyme Length | 461 |
| Uniprot Accession Number | Q9LTW4 |
| Absorption | |
| Active Site | ACT_SITE 124; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 338; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | ACTIVITY REGULATION: Repressed by pepstatin A. {ECO:0000269|PubMed:22987884}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Aspartic proteinase that can use azocasein as substrate and regulates endogenous sugar levels (e.g. sucrose, glucose and fructose) by modulating starch accumulation and remobilization (PubMed:22987884). Influences general morphology and development (PubMed:22987884). {ECO:0000269|PubMed:22987884}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6. {ECO:0000269|PubMed:22987884}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Chain (1); Domain (1); Erroneous initiation (1); Glycosylation (3); Transit peptide (1) |
| Keywords | Alternative splicing;Aspartyl protease;Chloroplast;Glycoprotein;Hydrolase;Plastid;Protease;Reference proteome;Transit peptide |
| Interact With | |
| Induction | INDUCTION: Expressed with a pronounced diurnal rhythm characterized by a peak at the end of the day and early night (at protein level). Slightly induced by sucrose (Suc). {ECO:0000269|PubMed:22987884}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000269|PubMed:22987884}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17142483; 17217462; 25752149; |
| Motif | |
| Gene Encoded By | |
| Mass | 50,568 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |