| IED ID | IndEnz0002015524 |
| Enzyme Type ID | protease015524 |
| Protein Name |
Myelin regulatory factor homolog 1 EC 3.4.-.- Polyglutamine-repeat protein 47 Cleaved into: Myelin regulatory factor homolog 1, N-terminal; Myelin regulatory factor homolog 1, C-terminal |
| Gene Name | myrf-1 pqn-47 F59B10.1 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MSSSDLLKGEFDGLNSEHFNMMQYLTQDTDEDDGSMVSPTSSADSMHQNLGVQQQQQQMLQAQQRQNQNGIFQPRRFPESPAMTDPCGNVSSSSSSSHHSDPMFSPNEFNGYAGANDNGNQTMNNIQSQQLSQQQHQQTRGGNLMMPQQSSIHAQMQNMNAPQFWSQPGTAAVNQPTNTLAQLNLFNIIRGGADSGMPSPVLEMPRKRSRLDTPCETPRIAPSFAGIDGFPDENYSQQQAIRFSKFQEEQWSPLYDINAQPLQQLQVHVVADKGFNYNSNDNCFVNQKKNHFQISVNVEASDTMPPKYVNFNNRLVPIRDFKLSFCGVKAEMPSSEITIRQSRADRKPHTHTPVLFEIQERRMTKVCVPRLHFSETTLNNQRKQKNRPNPEQKFFLLVVRLFASIDESEHGVLIQSYASEKVIVRATNPGSFEPQDTDIGWQRNGGALYTQGAVSVGTEHQVESAKLTVAGDIYMSGRIINPSDIRLKEAITERETAEAIENLLKLRVVDYRYKPEVADIWGLDEQQRHRTGLIAQELQAVLPDAVRDIGDYLTIDEGRVFYETVMATQQLCRMTGDLDSKIDEKVAEISRRLNEYAVRKKLASSMASNLNGDNKSLSYSRCSLTSTATNATSQPKRSRKHRAIKQAQSCGSRLSQGTVVTLVSIMAACLLAMSALYVLDWHNRNYGYHQHFETNTPSTKGELANLVISPANFMPSFQPDAPILLEKCFNPSCKTYCCTDTPPVVEDSRAIATHGLDNGDEVYPESPSNRTNGIARAPNLEHMAFETGVEIRIPALNVTLDQRYCVERSCNKRKGIFNVFVPVSRYMPDVALEIEIKAPISKVVSNCGAFPSTEFNHKVCPLSRTQQSESPVPTSTRLFDNIFELSMGSFIQSAYRFRVGYSTETCFSEDSNGSYEEYNLIFYRMCTLSSS |
| Enzyme Length | 931 |
| Uniprot Accession Number | G5EFI7 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 169..436; /note=NDT80; /evidence=ECO:0000255|PROSITE-ProRule:PRU00850 |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: [Myelin regulatory factor homolog 1]: Constitutes a precursor of the transcription factor (PubMed:28441531). Mediates the autocatalytic cleavage that releases the Myelin regulatory factor homolog 1, N-terminal component that specifically activates transcription of genes involved in synaptic rewiring during nervous system maturation (PubMed:28441531). {ECO:0000269|PubMed:28441531}.; FUNCTION: [Myelin regulatory factor homolog 1, C-terminal]: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage. {ECO:0000250|UniProtKB:Q9Y2G1}.; FUNCTION: [Myelin regulatory factor homolog 1, N-terminal]: Transcription factor that specifically activates expression of genes involved in synaptic rewiring during nervous system maturation (PubMed:28441531). Specifically required for dorsal D (DD) GABAergic motor neurons synaptic rewiring (PubMed:28441531, PubMed:33950834). Acts in complex with myrf-2 paralog (PubMed:28441531). {ECO:0000269|PubMed:28441531, ECO:0000269|PubMed:33950834}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Compositional bias (3); DNA binding (1); Domain (1); Glycosylation (2); Mutagenesis (7); Region (1); Site (1); Topological domain (2); Transmembrane (1) |
| Keywords | Activator;Autocatalytic cleavage;Cell membrane;Cytoplasm;DNA-binding;Differentiation;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Nucleus;Protease;Reference proteome;Transcription;Transcription regulation;Transmembrane;Transmembrane helix |
| Interact With | D9PTN5 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Myelin regulatory factor homolog 1]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:28441531}; Single-pass membrane protein {ECO:0000255}. Nucleus {ECO:0000269|PubMed:33950834}. Apical cell membrane {ECO:0000269|PubMed:33950834}; Single-pass membrane protein {ECO:0000255}. Note=In early L1 larvae, localizes to the cell membrane of the pharynx, epidermis, and neurons, but is not enriched in the nucleus or cytoplasm of these cell types (PubMed:33950834). Later in the L1 larval stage, accumulates in the nucleus of the pharynx, epidermis, and neurons (PubMed:33950834). {ECO:0000269|PubMed:33950834}.; SUBCELLULAR LOCATION: [Myelin regulatory factor homolog 1, N-terminal]: Endoplasmic reticulum membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. Nucleus {ECO:0000269|PubMed:28441531}. Cytoplasm {ECO:0000269|PubMed:28441531}. Apical cell membrane {ECO:0000269|PubMed:33950834}. Note=Translocates from the cytoplasm to the nucleus upon autocatalytic cleavage (PubMed:28441531). In early L1 larvae, localizes to the cell membrane of the pharynx, epidermis, and neurons, but is not enriched in the nucleus or cytoplasm of these cell types (PubMed:33950834). Cell membrane localization is promoted by pan-1 (PubMed:33950834). Later in the L1 larval stage, accumulates in the nucleus of the pharynx, epidermis, and neurons (PubMed:33950834). {ECO:0000269|PubMed:28441531, ECO:0000269|PubMed:33950834}.; SUBCELLULAR LOCATION: [Myelin regulatory factor homolog 1, C-terminal]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:28441531}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | PTM: Myelin regulatory factor: Follows autocatalytic cleavage via the peptidase S74 domain. Autoprocessing is apparently constitutive and is essential for transcriptional activity. {ECO:0000305|PubMed:28441531}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11231151; 12242258; 12529635; 14551910; 15338614; 15791247; 17164286; 17486083; 17850180; 19343510; 20439774; 20537990; 21085631; 21177967; 21367940; 22347378; 22500807; 22560298; 23791784; 23800452; 25487147; 26063786; 26430702; |
| Motif | |
| Gene Encoded By | |
| Mass | 104,463 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |