| IED ID | IndEnz0002015527 |
| Enzyme Type ID | protease015527 |
| Protein Name |
Myelin regulatory factor EC 3.4.-.- Myelin gene regulatory factor Cleaved into: Myelin regulatory factor, N-terminal; Myelin regulatory factor, C-terminal |
| Gene Name | Myrf Gm1804 Gm98 Mrf |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MEVVDETEALQRFFEGHDISGALEPSNIDTSILEEYIGKEDASDLCFPEISAPASTASFPHGPPAIPGSSGLHHLSPPGSGPSPGRHGPLPPPTYGTPLNCNNNNGMGTAPKPFLGGSGPPIKAEPKAPYAPGTLPDSPPDSGSEAYSPQQVNDPHLLRTITPETLCHVGVSSRLEHPPPPPAHLPGPPPPPPPPPHYPVLQRDLYMKAEPPVPPYAAMGPGLVPPELHHTQQTQVLHQLLQQHGAELPPHPSKKRKHSESPPNTLNAQMLNGMIKQEPGTVTALPPHPARAPSPPWPPQGPLSPGTGSLPLSIARAQTPPWHPPGAPSPGLLQDSDSLSGSYLDPNYQSIKWQPHQQNKWATLYDANYKELPMLTYRVDADKGFNFSVGDDAFVCQKKNHFQVTVYIGMLGEPKYVKTPEGLKPLDCFYLKLHGVKLEALNQSINIEQSQSDRSKRPFNPVTVNLPPEQVTKVTVGRLHFSETTANNMRKKGKPNPDQRYFMLVVALQAHAQNQNYTLAAQISERIIVRASNPGQFESDSDVLWQRAQLPDTVFHHGRVGINTDRPDEALVVHGNVKVMGSLMHPSDLRAKEHVQEVDTTEQLKRISRMRLVHYRYKPEFAASAGIEATAPETGVIAQEVKEILPEAVKDTGDVVFANGKTIENFLVVNKERIFMENVGAVKELCKLTDNLETRIDELERWSHKLAKLRRLDSLKSTGSSGAFSHAGSQFSRAGSVPHKKRPPKLANKSSPAVPDQACISQRFLQGTIIALVVVMAFSVVSMSTLYVLSLRSEEDLVDADGSLAVSTSCLLALLRPQDPGGSEAMCPWSSQSFGTTQLRQSSMTTGLPGTQPSLLLVTKSASGPALRALDLCSSQPCPIVCCSPPVSSPATDPALGPTLTPTPSPSSNPKHSGPGQMAPLPVTNIRAKSWGISANGISYSKHSKSLEPLASPVVPFPGGQSKTKNSPSFNLQSRARRGAPQPSPSPAQFTQTQGQLDPAPSLTSIQLLENSMPITSQYCVPEGACRLGNFTYHIPVSSSTPLHLSLTLQMNSSTPVSVVLCSLTSEEEPCEEGGFLQRFHPHQDTQGTSHQWPVTILSFREFTYHFRVTLLGQANCSSEAIVQPATDYYFHFYRLCD |
| Enzyme Length | 1138 |
| Uniprot Accession Number | Q3UR85 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 250..541; /note=NDT80; /evidence=ECO:0000255|PROSITE-ProRule:PRU00850 |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: [Myelin regulatory factor]: Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes. {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:28623291}.; FUNCTION: [Myelin regulatory factor, C-terminal]: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage. {ECO:0000269|PubMed:23966833}.; FUNCTION: [Myelin regulatory factor, N-terminal]: Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG, DUSP15 and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination (PubMed:19596243, PubMed:22956843, PubMed:23966833, PubMed:24204311, PubMed:27532821). Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype (PubMed:19596243, PubMed:22956843, PubMed:23966833). {ECO:0000269|PubMed:19596243, ECO:0000269|PubMed:22956843, ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:24204311, ECO:0000269|PubMed:27532821}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (5); Beta strand (17); Chain (3); Coiled coil (1); Compositional bias (4); DNA binding (1); Domain (1); Glycosylation (3); Helix (1); Modified residue (1); Motif (2); Mutagenesis (10); Region (8); Sequence conflict (1); Site (1); Topological domain (2); Transmembrane (1); Turn (1) |
| Keywords | 3D-structure;Acetylation;Activator;Alternative splicing;Autocatalytic cleavage;Coiled coil;Cytoplasm;DNA-binding;Differentiation;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Nucleus;Protease;Reference proteome;Transcription;Transcription regulation;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Expression is directly regulated by SOX10. {ECO:0000269|PubMed:24204311}. |
| Subcellular Location | SUBCELLULAR LOCATION: [Myelin regulatory factor]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:30249802}; Single-pass membrane protein.; SUBCELLULAR LOCATION: [Myelin regulatory factor, N-terminal]: Nucleus {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:28441531, ECO:0000269|PubMed:30249802}. Cytoplasm {ECO:0000269|PubMed:23966833}. Note=Translocates from the cytoplasm to the nucleus upon autocatalytic cleavage. {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:30249802}.; SUBCELLULAR LOCATION: [Myelin regulatory factor, C-terminal]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:30249802}; Single-pass membrane protein {ECO:0000269|PubMed:23966833}. |
| Modified Residue | MOD_RES 123; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:Q9Y2G1 |
| Post Translational Modification | PTM: [Myelin regulatory factor, C-terminal]: Glycosylated. {ECO:0000250|UniProtKB:Q9Y2G1}.; PTM: [Myelin regulatory factor]: Follows autocatalytic cleavage via the peptidase S74 domain. Autoprocessing is apparently constitutive and is essential for transcriptional activity (PubMed:23966833, PubMed:28623291, PubMed:30249802). Autocatalytic cleavage is inhibited by interaction with TMEM98 (PubMed:30249802). {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:28623291, ECO:0000269|PubMed:30249802}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5H5P; 7DC3; |
| Mapped Pubmed ID | 14610273; 17967808; 18799693; 21267068; 21938520; 22365546; 23332759; 23575864; 24257626; 25324381; 25568100; 25680202; 25789755; 26395480; 26525805; 26928066; 27149841; 27455109; 28160598; 28336932; 28695847; 29361054; 29472293; 29500351; 29556912; 30076300; 30166609; 30996143; 31048900; 31081019; 31753579; 31828317; 32042175; 32075970; 32236127; 32266943; 32270922; 32290105; 33472075; 33646289; 34345217; 34615523; |
| Motif | MOTIF 254..257; /note=Nuclear localization signal; /evidence=ECO:0000269|PubMed:23966833; MOTIF 491..494; /note=Nuclear localization signal; /evidence=ECO:0000269|PubMed:23966833 |
| Gene Encoded By | |
| Mass | 123,288 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |