| IED ID | IndEnz0002015528 |
| Enzyme Type ID | protease015528 |
| Protein Name |
Myelin regulatory factor EC 3.4.-.- Myelin gene regulatory factor Cleaved into: Myelin regulatory factor, N-terminal; Myelin regulatory factor, C-terminal |
| Gene Name | myrf mrf |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MDVEDENETLRRFFEGHDINGALEPSNIDTSILEDYISKEDSSEICFPDIPSSVSYASPQPCGSSGVHLSSVLSNVGHTGSINSGGVHHLGQQMAVRPGPGPTCGGPPYPPHLNCNNNNAMLNPKGYPMCMSNQGLPIKAEPKTSYAAGTLPDSPPDSGSEAYSPQQLSDPHLLRTMTPENICHITPPSRLEHPPPPHLQGPLPPHSIHQQHYPSMQRELYMKVESMMPQYQNLGPAMPPADLHHAQQSQMLHQLLQQQHGAELPVHPSKKRKHSDSPTNTLREQISNGGMVKSEPGLIQDNDSLNGSYLDPNYQSIKWQPHLQNKWVSLYDASYKELPMLTYKVDADKGFNFSTGDDSFVCQKKNHFQVTVYIGMIGEPKYVKTPEGILPIECFFLKLNGVKLEAINQAISIEQSQSDRSKRPFHPVTLSLPPDQVTKVTVGRLHFSETTSNNMRKKGKPNPDQRYFLLVVALQVQAQNQTYLVAAQASERIIVRASNPGQFESDSEVLWQRGQLPDTVFHHGRIGINTERPDEALVVHGNVKIMGSLMHPSDIRAKESVEEVDTTEQLKRISQMRLVHYHYKPEFASTVGLDENAAETGVIAQEVQEILPEAVKESGDLVCANGETIENFLVVNKERIFMENVGAVKELCKLTDNLETRIDELERWSHKLAKLRRLDSMKSTNSHTGSSQFSRAGSVPYKQRPPKVMGKTVPGPAHQSCVSQRFLQATIIALVIIMAFSVISMTTLYVLNLRSEDDMLGIDGSLTPPGSCTLTFFRQIHLTLPYALCTGSSQNFDTTQLKGNTTPPPKITKSPDWQQDPPLTINLCMDPPCEVVCCPHILSSESPTITRKTSAASAETISQTDPAPSTIIRKAKSRNLDKNRNSLQTLPRPVSPLPPYTQGKNKHSPNSLPVRDVRKRRSLEEESTPITPMDRTQGNSNDSRYSLTSLRLLETDALITNRSCSSMDTCGSGNYTYKLPISKYSPLSGSLSLELNSSSPVSVNFCETSKGKGCQEPAAVTSPRDQSCPQGTDVCVTQVSPTSHLWSLQLLPSQDFTFHLRVSPPGASGCDDLSIDPSQVTDYYFRFYRLCD |
| Enzyme Length | 1092 |
| Uniprot Accession Number | Q66IV1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 246..507; /note=NDT80; /evidence=ECO:0000255|PROSITE-ProRule:PRU00850 |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: [Myelin regulatory factor]: Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes (By similarity). {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}.; FUNCTION: [Myelin regulatory factor, C-terminal]: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage. {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}.; FUNCTION: [Myelin regulatory factor, N-terminal]: Transcription factor that specifically activates expression of myelin genes during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination. {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Coiled coil (1); Compositional bias (6); DNA binding (1); Domain (1); Glycosylation (4); Region (6); Site (1); Topological domain (2); Transmembrane (1) |
| Keywords | Activator;Autocatalytic cleavage;Coiled coil;Cytoplasm;DNA-binding;Differentiation;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Nucleus;Protease;Transcription;Transcription regulation;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Myelin regulatory factor]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}; Single-pass membrane protein {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}.; SUBCELLULAR LOCATION: [Myelin regulatory factor, N-terminal]: Nucleus {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}. Cytoplasm {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}. Note=Translocates from the cytoplasm to the nucleus upon autocatalytic cleavage. {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}.; SUBCELLULAR LOCATION: [Myelin regulatory factor, C-terminal]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}; Single-pass membrane protein {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}. |
| Modified Residue | |
| Post Translational Modification | PTM: [Myelin regulatory factor]: Follows autocatalytic cleavage via the peptidase S74 domain. Autoprocessing is apparently constitutive and is essential for transcriptional activity. {ECO:0000250|UniProtKB:Q3UR85, ECO:0000250|UniProtKB:Q9Y2G1}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 120,780 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |