| IED ID | IndEnz0002015555 |
| Enzyme Type ID | protease015555 |
| Protein Name |
Iron-regulated surface determinant protein A Fur-regulated protein A Staphylococcal transferrin-binding protein A |
| Gene Name | isdA frpA stbA SAOUHSC_01081 |
| Organism | Staphylococcus aureus (strain NCTC 8325 / PS 47) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus Staphylococcus aureus (strain NCTC 8325 / PS 47) |
| Enzyme Sequence | MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQVNAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK |
| Enzyme Length | 350 |
| Uniprot Accession Number | Q2FZE9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 75; /note=Heme; /evidence=ECO:0000269|PubMed:17229211; BINDING 82; /note=Heme; /evidence=ECO:0000269|PubMed:17229211 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Cell wall-anchored surface receptor that participates in the extraction of heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source (By similarity). Receives heme from IsdB and transfers it to IsdC (By similarity). Plays also a role in the inhibition of host immune response. Protects S.aureus against the bactericidal protease activity of apolactoferrin. Enhances bacterial cellular hydrophobicity, which renders S.aureus resistant to bactericidal human skin fatty acids as well as to beta-defensins and cathelicidin. Also binds fibronectin and chains B-beta and gamma of fibrinogen, promoting clumping of S.aureus with fibrinogen. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization (By similarity). {ECO:0000250|UniProtKB:A6QG31, ECO:0000250|UniProtKB:Q7A152}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (10); Binding site (2); Chain (1); Compositional bias (2); Domain (1); Helix (3); Metal binding (1); Modified residue (1); Motif (1); Mutagenesis (7); Propeptide (1); Region (1); Sequence conflict (13); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Cell wall;Direct protein sequencing;Heme;Iron;Metal-binding;Peptidoglycan-anchor;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Repressed by fur in the presence of iron. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250|UniProtKB:A6QG31}; Peptidoglycan-anchor {ECO:0000250|UniProtKB:A6QG31}. Note=Encodes an LPXTG motif-containing sorting signal that targets to the cell wall, which is catalyzed by sortase A. {ECO:0000250|UniProtKB:A6QG31}. |
| Modified Residue | MOD_RES 316; /note=Pentaglycyl murein peptidoglycan amidated threonine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00477 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..46; /evidence=ECO:0000250 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2ITE; 2ITF; |
| Mapped Pubmed ID | - |
| Motif | MOTIF 313..317; /note=LPXTG sorting signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00477 |
| Gene Encoded By | |
| Mass | 38,746 |
| Kinetics | |
| Metal Binding | METAL 166; /note="Iron (heme axial ligand)"; /evidence="ECO:0000269|PubMed:17229211, ECO:0007744|PDB:2ITE" |
| Rhea ID | |
| Cross Reference Brenda |