| IED ID |
IndEnz0002015571 |
| Enzyme Type ID |
protease015571 |
| Protein Name |
Antitoxin HigA
|
| Gene Name |
higA ygjM b3082 JW3053 |
| Organism |
Escherichia coli (strain K12) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Proteobacteria
Gammaproteobacteria
Enterobacterales
Enterobacteriaceae
Escherichia
Escherichia coli
Escherichia coli (strain K12)
|
| Enzyme Sequence |
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID |
| Enzyme Length |
138 |
| Uniprot Accession Number |
P67701 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
DNA_BIND 95..114; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00257 |
| EC Number |
|
| Enzyme Function |
FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system. Functions as an mRNA interferase antitoxin; overexpression prevents HigB-mediated cessation of cell growth and inhibition of cell proliferation. {ECO:0000269|PubMed:19943910}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); DNA binding (1); Domain (1); Helix (9); Turn (1) |
| Keywords |
3D-structure;DNA-binding;Reference proteome;Repressor;Toxin-antitoxin system;Transcription;Transcription regulation |
| Interact With |
P64578 |
| Induction |
INDUCTION: Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the higB-higA operon. {ECO:0000269|PubMed:19943910}. |
| Subcellular Location |
|
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
X-ray crystallography (4) |
| Cross Reference PDB |
5IFG;
6JQ4;
6KML;
6KMQ;
|
| Mapped Pubmed ID |
16606699;
24561554;
27601326;
31014676;
33000860;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
14,995 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|